Abstract: Objective To quarantine and identify the ticks carried by wool imported from South Africa to China, and to prevent the transmission of harmful parasites through international trade. Methods Thirty-one batches of wool imported from South Africa at Shanghai port from January 2016 to August 2018 were examined to detect ticks. The detected ticks were subjected to morphological identification. Genomic DNA was extracted from the ticks, and the mitochondrial 16S rRNA gene and cytochrome C oxidase subunit I gene were amplified and sequenced for molecular biological identification. Results Thirty-one batches of wool were imported from South Africa at Shanghai port from January 2016 to August 2018. Ticks were observed in 8 batches of the imported wool, with a positive detection rate of 25.8%. They belonged to 8 species and 3 genera as identified by morphological and molecular biological methods. There were 15 ticks of Rhipicephalus evertsi evertsi, as the predominant species (53.6% of the total number) of intercepted ticks carried by the wool imported from South Africa; there was no distribution record of this tick species in China. Conclusion The intercepted ticks carried by wool imported from South Africa are mainly Rh. e. evertsi, which can transmit multiple animal diseases including babesiosis.

Key words: South Africa, Wool, Rhipicephalus evertsi evertsi, Identification

摘要： 目的 为防止有害寄生虫通过国际贸易传入我国，对进口羊毛进行蜱监测与鉴定。方法 采集进口羊毛进行蜱检疫。将检出的蜱进行形态学鉴定，并提取其基因组DNA，扩增线粒体16S rRNA基因和细胞色素C氧化酶亚基Ⅰ（COⅠ）基因，并进行测序鉴定。结果 2016年1月至2018年8月上海口岸从南非进口的31批次羊毛中，有8批次羊毛检出蜱，阳性检出率为25.8%。经形态学和分子生物学鉴定，隶属3属8种，其中伊文斯扇头蜱15只，占截获蜱总数的53.6%，为本批次南非羊毛携带蜱的优势种类，该蜱在我国暂无分布记录。结论 从南非进口羊毛中检出的蜱主要为一种能传播巴贝斯虫病等多种动物疫病的伊文斯扇头蜱。

关键词: 南非, 羊毛, 伊文斯扇头蜱, 鉴定