An investigation of mosquitoes and mosquito-borne pathogens in the southwest border regions of Yunnan province, China

doi:10.11853/j.issn.1003.8280.2019.03.008

Chines Journal of Vector Biology and Control ›› 2019, Vol. 30 ›› Issue (3): 264-267.DOI: 10.11853/j.issn.1003.8280.2019.03.008

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An investigation of mosquitoes and mosquito-borne pathogens in the southwest border regions of Yunnan province, China

XIE Lyu¹, LIN An-qi², WANG Jian¹, WU Chao¹, LUO Yan³, YU Ai-shui³, CHEN Ran⁴, YANG Bo⁵, XIE Xiao-lu⁵, LI Chun-min¹, LI Zhi-jiao¹, ZHU Huai-min², JIANG Jin-yong¹

1 Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Academician Workstation of Professor Jin Ningyi, Yunnan Innovative Team of Key Techniques for Vector Borne Disease Control and Prevention(Developing), Training Base of International Scientific Exchange and Education in Tropical Diseases for South and Southeast Asia, Yunnan Provincial Center of Arborvirus Research, Yunnan Institute of Parasitic Disease, Pu′er 665000, Yunnan Province, China;
2 Department of Medical Microbiology and Parasitology, Naval Medical University;
3 Dai-Lu-Wa Autonomous County of Menglian Center for Disease Control and Prevention;
4 Menghai Center for Disease Control and Prevention;
5 Hani-Yi Autonomous County of Jiangcheng Center for Disease Control and Prevention

Received:2018-12-21 Online:2019-06-20 Published:2019-06-20
Supported by:
Supported by the National Key Research and Development Program of Biosafety Key Technology Research and Development (No. 2016YFC1200500)

云南省西南边境地区蚊虫及蚊媒病原体调查

谢吕¹, 林安琪², 王剑¹, 吴超¹, 罗燕³, 鱼爱水³, 陈然⁴, 杨波⁵, 谢晓露⁵, 李春敏¹, 李志娇¹, 朱淮民², 姜进勇¹

1 云南省虫媒传染病防控研究重点实验室, 金宁一院士工作站, 云南省虫媒传染病防控关键技术创新团队(培育), 面向南亚东南亚热带病国际科技人员交流与教育培训基地, 云南省虫媒病毒研究中心, 云南省寄生虫病防治所, 云南普洱 665000;
2 海军军医大学病原生物学教研室, 上海 200433;
3 孟连傣族拉祜族佤族自治县疾病预防控制中心, 云南孟连 665800;
4 勐海县疾病预防控制中心, 云南勐海 666200;
5 江城哈尼族彝族自治县疾病预防控制中心, 云南江城 665900

通讯作者: 姜进勇,Email:yipdjiang@126.com
作者简介:谢吕,男,医师,主要从事虫媒病毒疾病防治工作,Email:xielv2017@outlook.com
基金资助:
国家重点研发计划生物安全关键技术研发重点专项（2016YFC1200500）

Abstract

Abstract:

Objective To investigate the distribution of mosquito vectors and prevalence of mosquito-borne diseases in the southwest border regions of Yunnan province, China, and to provide a basis for the prevention and control of such diseases. Methods From 2013 to 2015, the methods of mosquito lamp and human landing collection were used to collect mosquitoes in Dai-Lahu-Va Autonomous County of Menglian, Menghai, and Hani-Yi Autonomous County of Jiangcheng of Yunnan. A kit was used to extract DNA and RNA, and RT-PCR and nucleotide sequencing were used to obtain the gene sequence of pathogens. Biological software including Clustal X1.83, DNASTAR 7.1, and MEGA 5.0 were used for nucleotide sequence analysis and phylogenetic analysis. Results A total of 126 893 mosquitoes of 39 species in 10 genera were collected, among which there were 104 476 Culex tritaeniorhynchus mosquitoes (82.33%), 18 422 Anopheles sinensis mosquitoes (14.52%), 1 212 Aedes albopictus mosquitoes (1.00%), and 891 Cx. pipiens quinquefasciatus mosquitoes (0.70%). Two strains of Japanese encephalitis virus (JEV) were isolated from Cx. tritaeniorhynchus, and Plasmodium, filaria, and other flaviviruses were not detected. Conclusion There are various vector mosquitoes in the border regions of Yunnan. Cx. tritaeniorhynchus and An. sinensis are dominant species in the local area. The isolated JEV suggest that the virus be prevalen in the southwest border regions of Yunnan, and therefore, the monitoring of key vector mosquitos should be enhanced.

Key words: Mosquito, Mosquito-borne disease, Pathogen, Border region, Yunnan province

摘要：

目的了解云南省西南边境地区蚊媒分布及相关蚊媒传染病的存在及流行情况，为该病的预防控制提供科学依据。方法 2013－2015年在云南省孟连傣族拉祜族佤族自治县、勐海和江城哈尼族彝族自治县采用诱蚊灯法和人工诱捕法进行蚊虫采集，采用试剂盒提取蚊虫DNA及RNA，通过反转录-聚合酶链式反应和核苷酸序列测定方法获得病原体基因序列，采用Clustal X1.83、DNASTAR 7.1和MEGA 5.0等生物学软件进行核苷酸序列分析及系统进化分析。结果共捕获成蚊10属39种126 893只，其中捕获三带喙库蚊104 476只、中华按蚊18 422只、白纹伊蚊1 212只、致倦库蚊891只，分别占82.33%、14.52%、1.00%和0.70%。分离出2株流行性乙型脑炎病毒（JEV），均来自三带喙库蚊，未检出疟原虫、丝虫及其他黄病毒。结论云南省边境地区蚊媒传染病媒介种类较多，三带喙库蚊和中华按蚊为优势蚊种，自然界中存在着JEV，应加强重要蚊媒监测工作。

关键词: 蚊虫, 蚊媒传染病, 病原体, 边境地区, 云南省

CLC Number:

XIE Lyu, LIN An-qi, WANG Jian, WU Chao, LUO Yan, YU Ai-shui, CHEN Ran, YANG Bo, XIE Xiao-lu, LI Chun-min, LI Zhi-jiao, ZHU Huai-min, JIANG Jin-yong. An investigation of mosquitoes and mosquito-borne pathogens in the southwest border regions of Yunnan province, China[J]. Chines Journal of Vector Biology and Control, 2019, 30(3): 264-267.

谢吕, 林安琪, 王剑, 吴超, 罗燕, 鱼爱水, 陈然, 杨波, 谢晓露, 李春敏, 李志娇, 朱淮民, 姜进勇. 云南省西南边境地区蚊虫及蚊媒病原体调查[J]. 中国媒介生物学及控制杂志, 2019, 30(3): 264-267.

References

[1] 查冰,周红宁,张海林,等. 云南省蚊虫媒介与蚊传虫媒病毒研究现状[J]. 中国媒介生物学及控制杂志,2012,23(5):439-444.
[2] 张海林. 云南省虫媒病毒研究进展[J]. 中国媒介生物学及控制杂志,2004,15(5):410-414. DOI:10.3969/j.issn.1003-4692. 2004.05.029.
[3] 杨杜鹃,付士红,张海林,等. 云南省东北等地区蚊虫及蚊媒病毒调查研究[J]. 中国媒介生物学及控制杂志,2011,22(4):304-308,312.
[4] 郭晓芳. 云南省澜沧江流域蚊虫及蚊媒病毒调查研究[D]. 北京:中国人民解放军军事医学科学院,2014.
[5] 李建雄,周兴武,赵晓涛,等. 云南省消除疟疾行动计划中期评估报告[J]. 中国热带医学,2016,16(4):343-348. DOI:10.13604/j.cnki.46-1064/r.2016.04.10.
[6] Guo XF,Yang HL,Wu C,et al. Molecular characterization and viral origin of the first Dengue outbreak in Xishuangbanna,Yunnan province,China,2013[J]. Am J Trop Med Hyg,2015,93(2):390-393. DOI:10.4269/ajtmh.14-0044.
[7] 刘永华,尹小雄,杨召兰,等. 云南省瑞丽市2013年登革热暴发的流行病学分析[J]. 中国媒介生物学及控制杂志,2014,25(6):524-526. DOI:10.11853/j.issn.1003.4692.2014.06.010.
[8] 李华昌,潘虹,冯云,等. 2015年云南省临沧市登革热暴发的流行病学调查[J]. 疾病监测,2016,31(7):561-565. DOI:10.3784/j.issn.1003-9961.2016.07.007.
[9] 卫生部疾病预防控制局. 登革热防治手册[M]. 北京:人民卫生出版社,2008:81-82.
[10] 中华人民共和国卫生部. 全国病媒生物监测方案(试行)[S]. 北京:中国疾病预防控制中心,2005.
[11] 高晓艳,付士红,邹文菁,等. 湖北省部分地区2010年蚊传虫媒病毒调查[J]. 中国媒介生物学及控制杂志,2015,26(2):133-136. DOI:10.11853/j.issn.1003.4692.2015.02.006.
[12] 冯云,李胜国,张海林,等. 云南腾冲县新分离乙型脑炎和盖塔病毒的分子生物学特征[J]. 中国人兽共患病学报,2014,30(4):353-357,363. DOI:10.3969/cjz.j.issn.1002-2694.2014. 04.003.
[13] 周正斌. 蚊媒病原体综合检测方法的建立及应用[D]. 上海:第二军医大学,2011.
[14] 王剑,姜进勇,郭晓芳,等. 中国-老挝边境地区蚊虫群落结构和地理生态位特征分析[J]. 中国媒介生物学及控制杂志,2017,28(3):209-215. DOI:10.11853/j.issn.1003.8280.2017. 03.004.
[15] 王丕玉,周红宁,吴超,等. 澜沧江下游地区蚊虫种类、分布及其孳生习性调查[J]. 中国热带医学,2011,11(6):657-659. DOI:10.13604/j.cnki.46-1064/r.2011.06.007.
[16] 张海林,自登云,施华芳,等. 云南省勐海县蚊虫及虫媒病毒调查报告[J]. 医学动物防制,1999,15(6):285-288.
[17] 张海林,自登云,龚正达,等. 云南省孟连县蚊虫调查及乙型脑炎病毒分离[J]. 医学动物防制,1998,14(2):9-11.
[18] 姜进勇,周红宁,郑宇婷,等. 云南三带喙库蚊对DDT和溴氰菊酯的抗药性研究[J]. 中国寄生虫学与寄生虫病杂志,2015,33(2):118-121.
[19] 刘欣. 中华按蚊常用杀虫剂的抗性研究[D]. 北京:中国疾病预防控制中心,2015.
[20] 兰学梅,郑宇婷,董朝良,等. 云南省瑞丽市埃及伊蚊和白纹伊蚊对常用杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志,2017,28(6):572-575. DOI:10.11853/j.issn.1003.8280. 2017.06.014.
[21] 郑学礼.我国蚊媒研究概况[J].中国病原生物学杂志,2014,9(02):183-187. DOI:10.13350/j.cjpb.10222.
[22] 成依依,周红宁.我国流行性乙型脑炎病毒基因型研究进展[J].中国病原生物学杂志,2018,13(12):1413-1415,1419.
[23] Wang HY,Takasaki T,Fu SH,et al. Molecular epidemiological analysis of Japanese encephalitis virus in China[J]. J Gen Virol,2007,88(3):885-894. DOI:10.1099/vir.0.82185-0.
[24] Li MH,Fu SH,Chen WX,et al. Genotype V Japanese encephalitis virus is emerging[J]. PLoS Negl Trop Dis,2011,5(7):e1231. DOI:10.1371/journal.pntd.0001231.
[25] Gao XY,Nasci R,Liang GD. The Neglected arboviral infections in mainland China[J]. PLoS Negl Trop Dis,2010,4(4):e624. DOI:10.1371/journal.pntd.0000624.
[26] 王静林,张海林,周济华,等. 云南省乙型脑炎病毒基因分型及分子流行病学研究[C]//中国畜牧兽医学会兽医公共卫生学分会成立大会暨第一次学术研讨会论文集. 上海:中国畜牧兽医学会,2008:1.
[27] 孙肖红. 我国云南省(滇)西北地区虫媒病毒调查[D]. 北京:中国疾病预防控制中心,2007.

An investigation of mosquitoes and mosquito-borne pathogens in the southwest border regions of Yunnan province, China

云南省西南边境地区蚊虫及蚊媒病原体调查

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