Genotypes of knockdown resistance gene and their distribution in Aedes albopictus in Haikou, China, in 2018

doi:10.11853/j.issn.1003.8280.2019.01.002

Chines Journal of Vector Biology and Control ›› 2019, Vol. 30 ›› Issue (1): 7-11.DOI: 10.11853/j.issn.1003.8280.2019.01.002

Previous Articles Next Articles

Genotypes of knockdown resistance gene and their distribution in Aedes albopictus in Haikou, China, in 2018

ZHAO Chun-chun¹, ZHU Cai-ying¹, KAI Wen-long¹, LIU Guo-jun², LIU Qi-yong¹, LIN Li³, QI Ai-ai³, WU Zhong-yi³, WANG Jun¹, SONG Xiu-ping¹, MENG Feng-xia¹

1 State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Vector Surveillance and Management, Beijing 102206, China;
2 Weifang Center for Disease Control and Prevention;
3 Hainan Health Pest Control Association

Received:2018-11-20 Online:2019-02-20 Published:2019-02-20
Supported by:
Supported by the Major Special Fund Projects of the 13th Five-Year Plan (No. 2017ZX10303404002005), National Science and Technology Major Project of China (No. 2018ZX10101002-011) and the National Natural Science Foundation of China (No. 81273139)

海口市2018年白纹伊蚊击倒抗性基因型分布研究

赵春春¹, 朱彩英¹, 开文龙¹, 刘国军², 刘起勇¹, 林丽³, 祁艾艾³, 吴钟毅³, 王君¹, 宋秀平¹, 孟凤霞¹

1 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, 世界卫生组织媒介生物监测与管理合作中心, 北京 102206;
2 潍坊市疾病预防控制中心, 山东潍坊 261061;
3 海南省卫生有害生物防制协会, 海南海口 570203

通讯作者: 孟凤霞,Email:mengfengxia@icdc.cn
作者简介:赵春春,女,在读硕士,主要从事蚊虫抗药性分子监测研究,Email:cczhaospring@163.com
基金资助:
“十三五”重大专项基金（2017ZX10303404002005）；国家科技重大专项（2018ZX10101002-011）；国家自然科学基金（81273139）

Abstract

Abstract: Objective To investigate the mutations in the knockdown resistance (kdr) gene of dengue vector Aedes albopictus in Haikou, China, as well as genotypes and their distribution in different areas, to analyze the influencing factors on insecticide resistance for scientific control of Ae. albopictus. Methods In June and July, 2018, the larvae and pupae of Ae. albopictus were collected from the urban and suburb areas of Haikou and reared to adults. The collected individuals were identified by morphology and were cryopreserved at -20℃ with anhydrous ethanol. DNA was extracted from a single mosquito, partial fragments of the voltage-gated sodium channel gene on nerve cell membrane were amplified by PCR, and sequencing was performed to analyze the mutations in the kdr gene. Results In the 94 Ae. albopictus mosquitoes in the east, south, west, north, and middle of Haikou, mutation was detected at the F1534 locus of the kdr gene. There were four alleles at the 1534 locus, namely, wild-type TTC/F (113/60.11%), mutant TCC/S (66/35.11%), mutant TCG/S (1/0.53%), and mutant TGC/C (8/4.26%); there were six genotypes, i.e., homozygous wild-type F/F (39/41.49%), wild/mutant heterozygote F/S (33/35.11%) and F/C (2/2.13%), homozygous mutant S/S (15/15.96%) and C/C (1/1.06%), and mutant heterozygote S/C (4/4.26%). The proportions of genotypes varied between populations. Conclusion The kdr mutation rate is high in Ae. albopictus in Haikou. Local mosquito resistance should be further monitored, so that insecticides are used appropriately.

Key words: Aedes albopictus, Insecticide resistance, Knockdown resistance gene, Gene mutation, Haikou

摘要： 目的了解海南省海口市登革热媒介白纹伊蚊的击倒抗性基因（kdr）在不同区域的基因型及其分布特点，并分析其抗药性产生的影响因素，为白纹伊蚊化学防控提供依据。方法 2018年6－7月在海口市东、南、西、北、中5个方位，采集白纹伊蚊幼虫及蛹，饲养至成蚊，经形态学方法鉴定后，用无水乙醇溶液浸泡，并于-20℃冻存备用。提取单只蚊虫的DNA，PCR扩增神经细胞膜上电压门控钠离子通道（VGSC）基因部分片段，测序后分析kdr基因突变情况。结果对海口市不同方位采集的94只白纹伊蚊的检测结果显示，其kdr基因F1534位点存在突变。1534位点共有4种等位基因，即野生型TTC/F（113/60.11%），突变型TCC/S（66/35.11%）、TCG/S（1/0.53%）和TGC/C（8/4.26%）；有6种基因型，分别为野生型纯合子F/F（39/41.49%），野生/突变型杂合子F/S（33/35.11%）、F/C（2/2.13%），突变型纯合子S/S（15/15.96%）、C/C（1/1.06%）和突变型杂合子S/C（4/4.26%）。各种群之间基因型比例各不相同。结论海口市kdr基因突变率较高，应进一步监测当地蚊虫抗药性，合理使用杀虫剂。

关键词: 白纹伊蚊, 抗药性, 击倒抗性基因, 基因突变, 海口市

CLC Number:

ZHAO Chun-chun, ZHU Cai-ying, KAI Wen-long, LIU Guo-jun, LIU Qi-yong, LIN Li, QI Ai-ai, WU Zhong-yi, WANG Jun, SONG Xiu-ping, MENG Feng-xia. Genotypes of knockdown resistance gene and their distribution in Aedes albopictus in Haikou, China, in 2018[J]. Chines Journal of Vector Biology and Control, 2019, 30(1): 7-11.

赵春春, 朱彩英, 开文龙, 刘国军, 刘起勇, 林丽, 祁艾艾, 吴钟毅, 王君, 宋秀平, 孟凤霞. 海口市2018年白纹伊蚊击倒抗性基因型分布研究[J]. 中国媒介生物学及控制杂志, 2019, 30(1): 7-11.

References

[1] 吴凡. 中国白纹伊蚊的分布和影响因素及登革热的风险评估研究[D]. 北京:中国疾病预防控制中心,2009.
[2] 杨舒然,刘起勇. 白纹伊蚊的全球分布及扩散趋势[J]. 中国媒介生物学及控制杂志,2013,24(1):1-4.
[3] 孟凤霞,王义冠,冯磊,等. 我国登革热疫情防控与媒介伊蚊的综合治理[J]. 中国媒介生物学及控制杂志,2015,26(1):4-10. DOI:10.11853/j.issn.1003.4692.2015.01.002.
[4] 陈翰明,高景鹏,姜进勇,等. 我国白纹伊蚊现场群体击倒抗性基因I1532和F1534突变检测及I1532T突变等位基因报告[J]. 中国媒介生物学及控制杂志,2018,29(2):120-125. DOI:10.11853/j.issn.1003.8280.2018.02.002.
[5] 姜进勇,陈辉莹,周红宁,等. 云南省三带喙库蚊对DDT和溴氰菊酯抗性群体的击倒抗性基因突变分析[J]. 中国寄生虫学与寄生虫病杂志,2017,35(6):536-540.
[6] 武松,马尔健,刘茜,等. 中华按蚊击倒抗性突变PCR检测方法的建立[J]. 中国人兽共患病学报,2011,27(11):1008-1010. DOI:10.3969/j.issn.1002-2694.2011.11.014.
[7] Kristan M,Abeku TA,Lines J. Effect of environmental variables and kdr resistance genotype on survival probability and infection rates in Anopheles gambiae (s.s.)[J]. Parasit Vectors,2018,11(1):560. DOI:10.1186/s13071-018-3150-8.
[8] Fernando SD,Hapugoda M,Perera R,et al. First report of V1016G and S989P knockdown resistant (kdr) mutations in pyrethroid-resistant Sri Lankan Aedes aegypti mosquitoes[J]. Parasit Vectors,2018,11(1):526. DOI:10.1186/s13071-018-3113-0.
[9] Gao JP,Chen HM,Shi H,et al. Correlation between adult pyrethroid resistance and knockdown resistance (kdr) mutations in Aedes albopictus (Diptera:Culicidae) field populations in China[J]. Infect Dis Poverty,2018,7(1):86. DOI:10.1186/s40249-018-0471-y.
[10] 王晓花,陈辉莹,杨新艳,等. 海口市白纹伊蚊对菊酯类杀虫剂的抗药性及击倒抗性基因突变分析[J]. 第二军医大学学报,2015,36(8):832-838. DOI:10.3724/SP.J.1008.2015.00832.
[11] 国务院办公厅. 国务院关于推进海南国际旅游岛建设发展的若干意见[国发[2009] 44号] [Z]. (2010-01-04)[2018-11-16]. http://www.gov.cn/zwgk/2010-01/04/content_1502531.htm.
[12] 吴维学,金玉明,孙莲英,等. 海南省2006年登革热传播媒介监测分析[J]. 中国热带医学,2007,7(10):1863-1864,1920. DOI:10.3969/j.issn.1009-9727.2007.10.071.
[13] 杜建伟,潘先海. 中国登革热流行概况与流行特征[J]. 中华流行病学杂志,2010,31(12):1429-1433. DOI:10.3760/cma.j.issn.0254-6450.2010.12.025.
[14] 罗永全,周艳华,王忠平,等. "双创"背景下海口市政府危机公关管理理念和能力建设的问题与对策研究[J]. 经济师,2017(1):173-176. DOI:10.3969/j.issn.1004-4914.2017.01.086.
[15] Kasai S,Ng LC,Lam-Phua SG,et al. First detection of a putative knockdown resistance gene in major mosquito vector,Aedes albopictus[J]. Jpn J Infect Dis,2011,64(3):217-221.
[16] 高景鹏,李凯利,陈辉莹,等. 菊酯类杀虫剂对海口市白纹伊蚊的效果测定[J]. 中国热带医学,2017,17(9):867-870. DOI:10.13604/j.cnki.46-1064/r.2017.09.04.
[17] Hasmiwati H,Rusjdi SR,Nofita E. Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang,Indonesia[J]. Biodiversitas,2018,19(1):31-36. DOI:10.13057/biodiv/d190105.
[18] Feng XY,Yang C,Yang YC,et al. Distribution and frequency of G119S mutation in Ace-1 gene within Anopheles sinensis populations from Guangxi,China[J]. Malar J,2015,14(1):470. DOI:10.1186/s12936-015-1000-0.
[19] Ishak IH,Riveron JM,Ibrahim SS,et al. The Cytochrome P450 gene CYP6P12 confers pyrethroid resistance in kdr-free Malaysian populations of the dengue vector Aedes albopictus[J]. Sci Rep,2016,6(1):24707. DOI:10.1038/srep24707.

Genotypes of knockdown resistance gene and their distribution in Aedes albopictus in Haikou, China, in 2018

海口市2018年白纹伊蚊击倒抗性基因型分布研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SUN Qin-tong, HAN Ying-nan, LIU Yan, WANG Xue-jun, LIU Feng, LAI Shi-hong, JING Xiao. Ecological investigation and analysis of Aedes albopictus， the vector of dengue fever， in Shandong province， China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 16-20.
[2]	LI Tian-qi, LIU Qin-mei, WU Yu-yan, LUO Ming-yu, WANG Jin-na, GONG Zhen-yu. Surveillance of the population density of Aedes albopictus larvae in dengue prone and risk areas of Zhejiang province, China, 2020 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 21-24.
[3]	WEI Chao, MA Hai-fang, LIAO Ning, XIONG Qi-meng. An analysis of special investigation results of dengue vector Aedes density in Guangxi Zhuang Autonomous Region， China， 2020-2021 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 25-29.
[4]	ZHAO Chun-chun, ZHOU Xin-xin, LI Wen-yu, LUN Xin-chang, LIU Xiao-bo, WU Hai-xia, WANG Jun, LIU Qi-yong, MENG Feng-xia. Insecticide resistance surveillance and characteristic analysis of dengue vector Aedes albopictus in 13 provinces of China in 2020 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 30-37.
[5]	ZHENG Yu-ting, YANG Chun-mei, YANG Ming-dong, JIANG Jin-yong. Ecological and insecticide resistance surveillance of dengue vector Aedes in Yunnan border region of China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 38-43.
[6]	TU Tao-tian, XIAO Han-sen, MENG Feng-xia, LIU Qi-yong, LIU Xiao-bo, HE Ya-ming, JI Heng-qing. An investigation of the insecticide resistance of Aedes vector after emergency control of a dengue fever outbreak in Chongqing， China， 2019 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 44-47.
[7]	LYU Wen-xiang, CHENG Peng, PENG Hui, WANG Hai-yang, WANG Hai-fang, GUO Xiu-xia, ZHANG Chong-xing, LIU Hong-mei, GONG Mao-qing, LIU Li-juan. Insecticide resistance of Culex pipiens pallens in Dongping Lake area， Shandong province， China，in 2021 [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 104-107.
[8]	ZHOU Xin-xin, LI Fen, DUAN Wen-bo, MA Xin-ran, SONG Xiu-ping, WU Shao-ying, MENG Feng-xia. Cloning and bioinformatics analysis of voltage-gated sodium channel gene of Aedes albopictus [J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 672-679.
[9]	XUE Hai-bin, MENG Jin-xia, REN Le, JING Xiao. Larval Aedes albopictus mosquito surveillance and risk assessment in Linyi, Shandong province, China, 2018-2020 [J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 686-690.
[10]	ZHANG Yu-fu, ZHANG Shou-gang, ZHU Ding, GU Deng-an, GE Xiao-wu, YUAN Jian-ming, CUI Qian, CHU Hong-liang. Distribution of Aedes albopictus in different floors of multi-story residential buildings in Jiangsu province, China [J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 700-704.
[11]	ZHOU Yi-bin, ZHU Yi-yi, ZHU Wei, YAO Jun-yi, ZHU Jiang, LENG Pei-en, WU Huan-yu. A study of spatial sampling methods for mosq-ovitrap monitoring [J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 749-755.
[12]	ZHU Wei, LIU Xiang-yu, ZHOU Yi-bin. Spatiotemporal variation of Aedes albopictus density treated by hedgerow spraying [J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 772-778.
[13]	ZHAO Qiong-yao, QIU Xing-hui, YANG Yan, LUO Xing, SONG Xiao-ming, JIA Yong-chao. Polymorphism and resistance mutation frequency of the target genes of insecticides in Anopheles sinensis in Guangyuan of Sichuan province, China [J]. Chinese Journal of Vector Biology and Control, 2021, 32(5): 541-545.
[14]	GUO Xiu-xia, CHENG Peng, LIU Li-juan, WANG Hai-fang, ZHANG Chong-xing, WANG Huai-wei, GONG Mao-qing. Cloning and sequence features of ultraviolet sensitive opsin gene in Aedes albopictus [J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 553-559.
[15]	ZHANG Jia-lin, HUANG Fa-yuan. Surveillance results of dengue vector Aedes in Anhui province of China, 2015-2019 [J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 582-585.