Application of DNA barcoding in species identification of bedbugs

doi:10.11853/j.issn.1003.8280.2018.05.009

Chines Journal of Vector Biology and Control ›› 2018, Vol. 29 ›› Issue (5): 458-461.DOI: 10.11853/j.issn.1003.8280.2018.05.009

Previous Articles Next Articles

Application of DNA barcoding in species identification of bedbugs

ZHANG Rui-ling^1,2, HUANG Zhen-dong¹, LIU Tong-kai¹, ZHAO Ai-hua³, ZHANG Zhong^1,2

1 Department of Pathogenic Biology of Taishan Medical University, Tai'an 271016, Shandong Province, China;
2 Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medical University;
3 Tai′an Center for Disease Control and Prevention

Received:2018-06-12 Online:2018-10-20 Published:2018-10-20
Supported by:
Supported by the National Natural Science Foundation of China (No. 81401693, 81572028)

DNA条形码技术在臭虫物种鉴定中的应用

张瑞玲^1,2, 黄振东¹, 刘同凯¹, 赵爱华³, 张忠^1,2

1 泰山医学院基础医学院病原生物学教研室, 山东泰安 271016;
2 泰山医学院新发传染病溯源及防控协同创新中心, 山东泰安 271016;
3 泰安市疾病预防控制中心, 山东泰安 271000

通讯作者: 张忠,Email:zhangz@tsmc.edu.cn
作者简介:张瑞玲,女,讲师,主要从事媒介生物学的分子系统学研究,Email:zhangrl_06@126.com
基金资助:
国家自然科学基金（81401693，81572028）

Abstract

Abstract: Objective To make accurate identification of bedbug species and provide information for implementation of effective prevention and control measures. Methods In this study, morphological identification and DNA barcoding that distinguishes species based on the differential of genetic distances of mitochondrial Cytochrome C oxidase subunit Ⅰgene (COⅠ), were employed to identify the bedbugs collected from a school of Tai'an in Shandong province. Results Firstly, length of pronotum is two times of width, corresponding with the description of Cimex lectularius. Secondly, genetic distances of unidentified bedbug samples with C. lectularius were 0-1.3%, while 27.6%-28.0% with C. hemipterus. Conclusion Both morphological characters and genetic distances supported that these bedbug samples belong to C. lectularius. Bedbugs are social insects, which is usually spread by human activities. Implementation of long term effective prevention and control measures are crucial for reducing and avoiding the threat caused by bedbugs.

Key words: Cimex lectularius, Cytochrome C oxidase subunit Ⅰ gene, Genetic distance

摘要： 目的准确鉴定臭虫样品，为制定和实施有效预防措施提供参考依据。方法联合利用形态学鉴定和基于线粒体细胞色素C氧化酶亚基Ⅰ（COⅠ）基因片段遗传距离的DNA条形码技术对山东省泰安市某学校发现的臭虫样品进行鉴定。结果臭虫样品的前胸背板宽约为身长的1/2，与温带臭虫的特征相符。遗传距离分析显示，实验样品与温带臭虫的遗传距离为0～1.3%，与热带臭虫的遗传距离为27.6%～28.0%。结论形态鉴定和DNA条形码分析结果均表明该实验样品为温带臭虫。臭虫通常是群居生活，且易于借助人类活动进行传播，长期、合理的防制是减少或避免臭虫危害的关键。

关键词: 温带臭虫, 细胞色素C氧化酶亚基Ⅰ基因, 遗传距离

CLC Number:

Q78
R384.3

ZHANG Rui-ling, HUANG Zhen-dong, LIU Tong-kai, ZHAO Ai-hua, ZHANG Zhong. Application of DNA barcoding in species identification of bedbugs[J]. Chines Journal of Vector Biology and Control, 2018, 29(5): 458-461.

张瑞玲, 黄振东, 刘同凯, 赵爱华, 张忠. DNA条形码技术在臭虫物种鉴定中的应用[J]. 中国媒介生物学及控制杂志, 2018, 29(5): 458-461.

References

[1] 陆宝麟,吴厚永. 中国重要医学昆虫分类与鉴别[M]. 郑州:河南科学技术出版社,2003:618-619.
[2] 李朝品. 医学节肢动物学[M]. 北京:人民卫生出版社,2009:752-755.
[3] Goddard J,deShazo R. Bed bugs (Cimex lectularius) and clinical consequences of their bites[J]. JAMA,2009,301(13):1358- 1366. DOI:10.1001/jama.2009.405.
[4] Potter MF. The history of bed bug management-with lessons from the past[J]. Am Entomol,2011,57(1):14-25. DOI:10.1093/ae/57.1.14.
[5] Kilpinen O,Jensen KMV,Kristensen M. Bed bug problems in Denmark,with a European perspective[C]//Robinson WH,Bajomi D. Proceedings of the Sixth International Conference on Urban Pests. Budapest,Hungary:International Conference on Urban Pests,2008:395-399.
[6] 许荣满. 臭虫危害的复燃和防治[J]. 中华卫生杀虫药械,2010,16(5):398-399.
[7] 薄景信,朱世营,杨晨光,等. 一艘入境船舶携带臭虫的调查及处置[J]. 中国媒介生物学及控制杂志,2013,24(5):462-463,466. DOI:10.11853/j.issn.1003.4692.2013.05.025.
[8] 王秀云,贺洪国,张淑群,等. 一起臭虫叮咬人事件调查[J]. 中国媒介生物学及控制杂志,2014,25(4):372. DOI:10.11853/j.issn.1003.4692.2014.04.026.
[9] 李兴文,马涛,王蓬,等. 深圳地区臭虫侵害调查研究[J]. 中华卫生杀虫药械,2013,19(3):236-238,241.
[10] Tamura K,Dudley J,Nei M,et al. MEGA4:molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Mol Biol Evol,2007,24(8):1596-1599. DOI:10.1093/molbev/msm092.
[11] Hebert PDN,Cywinska A,Ball SL,et al. Biological identifications through DNA barcodes[J]. Proc R Soc Lond B Biol Sci,2003,270(1512):313-321. DOI:10.1098/rspb.2002.2218.
[12] Angelakis E,Socolovschi C,Raoult D. Bartonella quintana in Cimex hemipterus,Rwanda[J]. Am J Trop Med Hyg,2013,89(5):986-987. DOI:10.4269/ajtmh.13-0182.
[13] Cooper R,Wang CL,Singh N. Mark-release-recapture reveals extensive movement of bed bugs (Cimex lectularius L.) within and between apartments[J]. PLoS One,2015,10(9):e0136462. DOI:10.1371/journal.pone.0136462.
[14] Saenz VL,Booth W,Schal C,et al. Genetic analysis of bed bug populations reveals small propagule size within individual infestations but high genetic diversity across infestations from the eastern United States[J]. J Med Entomol,2012,49(4):865-875. DOI:10.1603/ME11202.
[15] Singh N,Wang CL,Wang DS,et al. Comparative efficacy of selected dust insecticides for controlling Cimex lectularius (Hemiptera:Cimicidae)[J]. J Econ Entomol,2016,109(4):1819-1826. DOI:10.1093/jee/tow129.

Application of DNA barcoding in species identification of bedbugs

DNA条形码技术在臭虫物种鉴定中的应用

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 6

Recommended Articles

Metrics

Comments

[1]	LI Xin-mei, SUN Meng-tao, ZHAN Yu-juan, ZHANG Lan-xiang, LI Meng-zhu, TAO Dong-dong, SUN En-tao. Molecular and morphological identification of Cheyletus malaccensis and C. carnifex [J]. Chines Journal of Vector Biology and Control, 2021, 32(2): 230-234.
[2]	JIA Xiao-kai, GAO Chun-hua, ZHOU Dan-dan, WEI Fu-rong, LIU Qi-yong, REN Dong-sheng. Measurement of the median lethal dose of several common insecticides against Cimex lectularius [J]. Chines Journal of Vector Biology and Control, 2020, 31(1): 46-48.
[3]	LIU Nian, ZHOU Xiao-jie, QI Guang-hao, LI Mei, QIU Xing-hui. Detection of knockdown resistance mutations in a Cimex lectularius field population in Beijing, China [J]. Chines Journal of Vector Biology and Control, 2019, 30(5): 524-527.
[4]	GUO Li-min, XI Jin-xiao, GAI Yong-zhi, WU Bin, WANG Shi-ming, MIAO Ke-jun, MU Tao-xia, XU Da-qin. Application of DNA barcoding in the identification of rodent species in plague foci in Gansu province, China [J]. Chines Journal of Vector Biology and Control, 2019, 30(2): 176-179.
[5]	MA Fei-ya, MEI Lin, ZHANG Ya-fen, WANG Hui-yu, LIU Jia-jia, HAN Xue-qing. A comparative analysis of the sequence of cytochrome C oxidase subunit I gene in Ixodes persulcatus from Heilongjiang and Jilin ports and foreign countries [J]. Chines Journal of Vector Biology and Control, 2019, 30(1): 47-49.
[6]	Fu Wan-zhen;Yin Kai-shong;et al. Clinical Study of the RelationshiP Between the Bed Bug and Allergic Asthma [J]. Chines Journal of Vector Biology and Control, 1995, 06(1): 54-57.