Safety evaluation of Beauveria bassiana CF08 strain on Nasonia vitripennis

doi:10.11853/j.issn.1003.8280.2018.03.008

Abstract

Abstract: Objective To evaluate the safety of Beauveria bassiana CF08 strain on the fly pupal parasitoid, Nasonia vitripennis. Methods Insect-dipping test and spore-feeding method were used to evaluate the safety of B. bassiana CF08 strain on N. vitripennis larvae and adults. Results After spore-dipping or spore-feeding, the longevities of N. vitripennis female adults were (11.09±0.15)and (10.92±0.15)d, the control group was about (10.99±0.19)d, there was no significant difference among them (F=0.893, P=0.446). The longevity of spore-dipped, spore-fed and control male parasitoids were (5.83±0.09), (5.88±0.03), and (5.75±0.09)d, respectively. There was also no significant difference among the three groups (F=2.614, P=0.152). When the parasitized fly pupae dipped in the spore suspensions of B. bassiana CF08 strain, the parasitoid emergence rate, number of parasitoids emerged per fly pupa and sex ratio were more than 80%, 43 and 90%, respectively. The three indicators of spore-dripping groups and control group had no significant difference. Nasonia vitripennis emerged from spore-dripping fly pupae and control pupae had the same parasitic capacity to B. peregrine pupae. The parasitic rate, parasitoid emergence rate, number of parasitoids emerged from per fly pupa and sex ratio were more than 90%, 80%, 43 and 90%, respectively. There was also no significant difference among the spore-dripping groups and control group. Conclusion Beauveria bassiana CF08 strain had no negative effects on the development of immature stage and longevity of N. vitripennis adults. Exposure to B. bassiana spores at immature stage had no significant effects on the offspring quality of N. vitripennis. The parasitoid and entomogenous fungus could be used in combination for biological control of flies.

Key words: Beauveria bassiana, Nasonia vitripennis, Safety, Biological control

摘要： 目的研究球孢白僵菌CF08株对丽蝇蛹集金小蜂的安全性。方法采用浸虫法和饲喂法研究球孢白僵菌CF08株对丽蝇蛹集金小蜂成虫的安全性；采用浸渍法研究球孢白僵菌CF08株对丽蝇蛹集金小蜂幼期的安全性。利用SPSS 20.0软件进行统计学分析，组间比较采用单因素方差分析，多重比较采用Duncan法。结果浸虫法和饲喂法处理后丽蝇蛹集金小蜂雌蜂寿命分别为（11.09±0.15）和（10.92±0.15） d，对照组雌蜂寿命为（10.99±0.19） d，三者间差异无统计学意义（F=0.893，P=0.446）；浸虫法和饲喂法雄蜂寿命分别为（5.83±0.09）和（5.88±0.03） d，对照组雄蜂寿命为（5.75±0.09） d，三者间差异亦无统计学意义（F=2.614，P=0.152）。被寄生不同时间的棕尾别麻蝇蛹浸渍球孢白僵菌孢子液后，蝇蛹中丽蝇蛹集金小蜂的出蜂率 > 80%，平均出蜂数为43只/蛹，雌蜂比 > 90%，羽化出的丽蝇蛹集金小蜂雌雄蜂体长平均为2.0和1.4 mm，与对照组比较差异无统计学意义（P > 0.05）。羽化出的雌蜂对棕尾别麻蝇蛹的寄生率均 > 90%，出蜂率 > 80%，平均出蜂数约为43只/蛹，后代的雌蜂比 > 90%，与对照组比较差异均无统计学意义（P > 0.05）。结论幼期暴露球孢白僵菌CF08株对丽蝇蛹集金小蜂的生长、发育、繁殖和后代品质均无显著影响，两者可联合应用于蝇类的生物防治。

关键词: 球孢白僵菌, 丽蝇蛹集金小蜂, 安全性, 生物防治

CLC Number:

S476⁺.3

HUANG Zhen-dong, WAN Qing, ZHUANG Gui-fen, XUE Zhi-jing, ZHANG Rui-ling, ZHANG Zhong. Safety evaluation of Beauveria bassiana CF08 strain on Nasonia vitripennis[J]. Chines Journal of Vector Biology and Control, 2018, 29(3): 250-253.

黄振东, 万晴, 庄桂芬, 薛志静, 张瑞玲, 张忠. 球孢白僵菌CF08株对丽蝇蛹集金小蜂的安全性评价[J]. 中国媒介生物学及控制杂志, 2018, 29(3): 250-253.

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