Abstract: Objective To understand the resistance of main vectors to commonly-used insecticides by the successive investigation in Tangshan International Horticultural Exposition park and surrounding areas from 2012 to 2016, and to provide the scientific basis for vector control. Methods Using dipping, topical application and residual film methods to measure the resistance of Culex pipiens pallens, Musca domestica, and Blattella germanica. Results The resistance ratio of Cx. pipiens pallens to temephos, chlorpyrifos, cypermethrin, tetramethrin and DDT were 0.82, 0.50, 1.94, 2.18, and 0.88, and all considered susceptible. As compared with the previously recorded results(1983, 1991), however, Cx. pipiens pallens showed increased tolerance to deltamethrin in 2009-2011. The resistance ratio of M. domestica to tetramethrin, beta-cyfluthrin, alpha-cypermethrin, deltamethrin, and azamethiphos were 3.13, 2.21, 7.07, 100.67, and 6.71, respectively. Musca domestica had low resistance to tetramethrin, beta-cyfluthrin, alpha-cypermethrin, and azamethiphos, and with high resistance to deltamethrin. As compared with the previously reported results(1983), M. domestica showed increased resistance to deltamethrin in 2012-2016,and slightly decreased compared with 1991. The resistance ratio of B. germanica to cypermethrin, deltamethrin, tetramethrin, beta-cyfluthrin, and chlorpyrifos were 37.28, 18.61, 35.95, 27.05, and 17.04, and all considered high resistance. Conclusion Choosing high sensitive pesticides to reasonably kill vectors will ensure effective prevention and control of the vectors for 2016 International Horticultural Exposition.

Key words: International Horticultural Exposition, Vector, Insecticide resistance

摘要： 目的 调查河北省唐山市世界园艺博览会（世园会）园区及周边区域主要病媒生物对常用杀虫剂的抗药性，为唐山世园会科学使用杀虫剂、有效防制病媒生物提供科学依据。方法 2012-2016年采用浸渍法、点滴法和药膜法对唐山市世园会园区及周边区域的淡色库蚊、家蝇和德国小蠊进行抗药性测定。结果 唐山市淡色库蚊对双硫磷、毒死蜱、氯氰菊酯、胺菊酯和DDT的抗性倍数分别为0.82、0.50、1.94、2.18和0.88倍，抗性等级均为敏感，2009-2011年淡色库蚊对溴氰菊酯的抗药性与1983、1991年比较明显增强。家蝇对胺菊酯、高效氟氯氰菊酯、顺式氯氰菊酯、溴氰菊酯和甲基吡恶磷的抗性倍数分别为3.13、2.21、7.07、100.67和6.71倍，除对溴氰菊酯为高抗外，对其他杀虫剂均为低抗；1991年家蝇对溴氰菊酯的抗药性与1983年比较明显升高，至2012-2016年其抗性倍数呈下降趋势。德国小蠊对氯氰菊酯、溴氰菊酯、胺菊酯、高效氟氯氰菊酯和毒死蜱的抗性倍数分别为37.28、18.61、35.95、27.05和17.04倍，其抗性等级均为高抗。结论 唐山市针对世园会开展的病媒生物抗药性调查研究，选用敏感性较高的杀虫剂对病媒生物进行合理地杀灭，为2016年世园会有效防制病媒生物提供了理论指导。

关键词: 世界园艺博览会, 病媒生物, 抗药性