Abstract: Objective To determine the effect of diflubenzuron on dengue vector Aedes mosquitoes and provide evidence for mosquito resistance management and vector control. Methods WHO recommended method for insect growth regulators was utilized; the resistant Aedes strains were collected from Jinghong city the dengue fever epidemic area, the data were analyzed with SPSS 24.0 to create a log-probit model to determine the IE₅₀ of the susceptible strains and resistant strains, calculate the resistance ratio, observe the the stage-and concentration-dependent mortality, and analyze the characteristics of diflubenzuron against Aedes mosquitoes. Results The IE_50s of the resistant strains of Ae. aegypti and Ae. albopictus were 0.002 53(95%CI:0.002 16-0.002 89) μg/ml and 0.002 33(95%CI:0.001 98-0.003 12) μg/ml, the resistant ratios were 1.77-and 1.71-fold, indicating that no cross resistance between diflubenzuron and pyrethroid. The mortality peak of Ae. albopictus and Ae. aegypti was 4-6 d post-exposure, indicating that the effect of diflubenzuron was slow as compared with commonly used conventional insecticides. Under the different concentrations, the effect was different. The low concentration mainly resulted mortality at adult stage, whilethe high concentration mostly impacted on pupal and/or larval stages. Conclusion The resistant strains of Ae. albopictus and Ae. aegypti were susceptible to diflubenzuron. The recommended dose was 0.006 μg/ml,but the lethal effect of diflubenzuron was slow. It can be used as a selective insecticide for resistance management and vector control.

Key words: Dengue fever, Aedes aegypti, Aedes albopictus, Drug insecticide, Diflubenzuron

摘要： 目的 了解除虫脲对登革热媒介埃及伊蚊和白纹伊蚊的作用效果，为蚊虫抗药性治理和蚊媒控制提供依据。方法 用WHO推荐的昆虫生长调节剂的抗药性测定方法，以采自景洪市登革热疫区的埃及伊蚊和白纹伊蚊及实验室敏感品系为研究对象，研究除虫脲对这2种伊蚊的作用效果及其特点；用SPSS 24.0软件进行概率回归分析，得到除虫脲对伊蚊敏感品系和抗性种群的半数抑制剂量（IE₅₀），计算抗性倍数；通过观察不同浓度除虫脲作用下伊蚊的虫态构成及死亡情况，分析除虫脲对伊蚊的作用特点。结果 景洪市埃及伊蚊和白纹伊蚊拟除虫菊酯类抗性种群对除虫脲的IE₅₀及95%CI分别为0.002 33（0.001 98~0.003 12）和0.002 53（0.002 16~0.002 89）μg/ml，其抗性倍数分别为1.77和1.71倍，属于敏感平，除虫脲与拟除虫菊酯类药物无交互抗性；使用除虫脲后，埃及伊蚊和白纹伊蚊的死亡高峰均在第4~6天，表明与常用的化学杀虫剂比较，除虫脲致死作用比较缓慢；不同浓度除虫脲对蚊虫作用效果不同，低浓度主要在成蚊阶段死亡，高浓度均出现蛹和/或幼虫死亡。结论 景洪市埃及伊蚊和白纹伊蚊抗性种群对除虫脲均敏感，推荐使用剂量为0.006 μg/ml，但其致死作用比较缓慢，适用于蚊虫的抗性治理和可持续控制。

关键词: 登革热, 埃及伊蚊, 白纹伊蚊, 抗药性, 除虫脲