Abstract: Objective To understand the dynamic changes of trehalose and trehalase of Anopheles sinensis. Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect trehalose and trehalase contents of female adult. ANOVA was used to determine the statistical significance of trehalose and trehalase contents. Subsequently, Tukey's multiple comparison was applied for significance analysis. Results The results showed that the trehalose and trehalase of female An. sinensis increased first and decreased there after post adult emergence. The females after oviposition had lower levels of trehalose and trehalase contents. The contents of trehalose which reached the peak approximately on the second day in adult stage[(2.658 5±0.116 5) mg/g] were significantly different (F=21.383, df=100, P<0.05). However, the contents of trehalase reached the peak stage approximately on the third day in adult stage[(0.111 6±0.002 5) μmol/g]. Conclusion The trehalose and trehalase contents of female An. sinensis have a regular pattern, which is related to the energy accumulation and consumption.

Key words: Anopheles sinensis, Trehalose, Trehalase, Mosquito-borne infectious diseases

摘要： 目的 了解中华按蚊雌成蚊体内海藻糖及海藻糖酶含量的变化规律。方法 利用ELISA测定羽化不同时间的中华按蚊雌成蚊体内的海藻糖及海藻糖酶含量，利用单因素方差和Tukey多重比较方法进行统计学分析。结果 中华按蚊体内海藻糖及海藻糖酶含量随羽化时间的延长先增加后减少，成蚊产卵后，体内海藻糖及海藻糖酶含量低于吸血前。海藻糖含量在羽化第2天达最大值[（2.658 5±0.116 5）mg/g]，不同羽化时间海藻糖含量差异有统计学意义（F=21.383，df=100，P<0.05）；海藻糖酶在羽化第3天达最大值[（0.111 6±0.002 5）μmol/g]。结论 中华按蚊雌成蚊体内海藻糖及海藻糖酶含量在羽化后均呈规律性变化，可能与蚊虫能量的积累和消耗有关。

关键词: 中华按蚊, 海藻糖, 海藻糖酶, 蚊媒传染病