Investigation on the resistance of Aedes aegypti and Ae. albopictus to several insecticides in Ruili city, Yunnan province

doi:10.11853/j.issn.1003.8280.2017.06.014

Chines Journal of Vector Biology and Control ›› 2017, Vol. 28 ›› Issue (6): 572-575.DOI: 10.11853/j.issn.1003.8280.2017.06.014

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Investigation on the resistance of Aedes aegypti and Ae. albopictus to several insecticides in Ruili city, Yunnan province

LAN Xue-mei¹, ZHENG Yu-ting¹, DONG Chao-liang², LIU Yong-hua³, YIN Xiao-xiong³, YANG Ming-dong¹, JIANG Jin-yong¹

1 Yunnan Institute of Parasitic Diseases, Yunnan Center of Arboviruses Research, Yunnan Key Laboratory of Insect-borne Infectious Disease Control and Prevention, Yunnan Center for Public Health and Disease Prevention and Control of Collaborative Innovation, Pu′ er 665099, Yunnan Province, China;
2 Dehong Prefecture Center for Disease Control and Prevention;
3 Ruili Center for Disease Control and Prevention

Received:2017-06-25 Online:2017-12-20 Published:2017-12-20
Supported by:
Supported by the National Basic Research Program of China(No. 2016YFC1200500)

云南省瑞丽市埃及伊蚊和白纹伊蚊对常用杀虫剂的抗药性调查

兰学梅¹, 郑宇婷¹, 董朝良², 刘永华³, 尹小雄³, 杨明东¹, 姜进勇¹

1 云南省寄生虫病防治所, 云南省虫媒病毒研究中心, 云南省虫媒传染病防控研究重点实验室, 云南省公共卫生与疾病防控协同创新中心, 云南普洱 665099;
2 德宏州疾病预防控制中心, 云南芒市 678499;
3 瑞丽市疾病预防控制中心, 云南瑞丽 678600

通讯作者: 姜进勇,Email:yipdjiang@126.com;杨明东,Email:quanjueyang@163.com
作者简介:兰学梅,女,医师,从事媒介生物学与控制研究,Email:lanxuemei0715@163.com
基金资助:
国家重点研发计划（2016YFC1200500）

Abstract

Abstract: Objective To investigate the resistance of Aedes aegypti and Ae. albopictus to insecticides in Ruili city, Yunnan province, to provide the basis for integrated control of dengue vector. Methods Larvae of Ae. aegypti and Ae. albopictus were collected in Ruili city and Jiegao port and reared in laboratory to get F1 generation, to test their susceptibility to different kinds of insecticides by bioassays. The resistance level was decided by adjusted mortality. Results The 24 h mortality rate of adult Ae. aegypti in Ruili city to 0.25% permethrin, 0.03% λ-cyhalothrin, 8.00% α-cypermethrin, 0.80%malathion, 0.25% fenitrothion, 0.10% propoxur, and 4.00% DDT were 1.40% (resistant), 20.12% (resistant), 80.31% (suspicious resistant), 100% (susceptible),100% (susceptible), 100% (susceptible) and 14.93% (resistant)respectively. The 24 h mortality rate of adult Ae. aegypti in Jiegao port to above insecticides were 0.55% (resistant), 2.13% (resistant), 62.35% (resistant), 100% (susceptible), 100% (susceptible), 100% (susceptible) and 0 (resistant) respectively. In addition, the 24 h mortality of adult Ae. albopictus in Ruili city to 0.75% permethrin, 1.80% λ-cyhalothrin, 8.00% α-cypermethrin, 0.10% deltamethrin, 3.00% beta-cypermethrin, 0.44% malathion, 0.18% fenitrothion and 0.06% propoxur were 8.11% (resistant), 83.85% (suspicious resistant), 75.18% (resistant), 4.97% (resistant), 89.13% (suspicious resistant), 99.23% (susceptible), 100% (susceptible) and 100% (susceptible) respectively. Conclusion Aedes aegypti and Ae. albopictus in Ruili city have developed different levels resistance to pyrethroid insecticides, but present high susceptible to malathion, fenitrothion and propoxur.

Key words: Aedes aegypti, Aedes albopictus, Bioassay, Insecticide resistance, Ruili city

摘要： 目的掌握云南省瑞丽市埃及伊蚊和白纹伊蚊对常用杀虫剂的抗药性水平，为登革热媒介伊蚊综合治理提供依据。方法于2016年6－9月分别从云南省瑞丽市城区和姐告口岸采集埃及伊蚊和白纹伊蚊蛹或幼虫，实验室繁殖至子一代（F1），采用成蚊滤纸接触筒法，测定其对常用杀虫剂的抗药性，判定其抗性程度。结果瑞丽市城区埃及伊蚊对0.25%氯菊酯、0.03%氯氟氰菊酯、8.00%顺式氯氰菊酯、0.80%马拉硫磷、0.25%杀螟硫磷、0.10%残杀威和4.00% DDT的24 h后校正死亡率分别为1.40%（抗性）、20.12%（抗性）、80.31%（可疑抗性）、100%（敏感）、100%（敏感）、100%（敏感）和14.93%（抗性）；姐告口岸埃及伊蚊对上述杀虫剂24 h后校正死亡率分别为0.55%（抗性）、2.13%（抗性）、62.35%（抗性）、100%（敏感）、100%（敏感）、100%（敏感）和0（抗性）。另外，瑞丽市城区白纹伊蚊对0.75%氯菊酯、1.80%氯氟氰菊酯、8.00%顺式氯氰菊酯、0.10%溴氰菊酯、3.00%高效氯氰菊酯、0.44%马拉硫磷、0.18%杀螟硫磷和0.06%残杀威的24 h后校正死亡率分别为8.11%（抗性）、83.85%（可疑抗性）、75.18%（抗性）、4.97%（抗性）、89.13%（可疑抗性）、99.23%（敏感）、100%（敏感）和100%（敏感）。结论瑞丽市埃及伊蚊和白纹伊蚊对拟除虫菊酯类杀虫剂均产生了不同程度的抗药性；对有机磷类和氨基甲酸酯类杀虫剂仍敏感。

关键词: 埃及伊蚊, 白纹伊蚊, 生物学测定, 抗药性, 瑞丽市

CLC Number:

LAN Xue-mei, ZHENG Yu-ting, DONG Chao-liang, LIU Yong-hua, YIN Xiao-xiong, YANG Ming-dong, JIANG Jin-yong. Investigation on the resistance of Aedes aegypti and Ae. albopictus to several insecticides in Ruili city, Yunnan province[J]. Chines Journal of Vector Biology and Control, 2017, 28(6): 572-575.

兰学梅, 郑宇婷, 董朝良, 刘永华, 尹小雄, 杨明东, 姜进勇. 云南省瑞丽市埃及伊蚊和白纹伊蚊对常用杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2017, 28(6): 572-575.

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Investigation on the resistance of Aedes aegypti and Ae. albopictus to several insecticides in Ruili city, Yunnan province

云南省瑞丽市埃及伊蚊和白纹伊蚊对常用杀虫剂的抗药性调查

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