Abstract: Objective To investigate the insecticide resistance of Musca domestica in recent years to several common insecticides in Wuhan city, China, and to provide a scientific basis for the control of flies. Methods Musca domestica was collected in the urban area of Wuhan city and raised in the laboratory for 1-2 generations. WHO-recommended topical application for housefly was used to test medium lethal dose of M. domestica in 3-4 days. Results The LD₅₀ of DDVP, azamethiphos chlorpyrifos, deltamethrin and beta cypermethrin was 0.854 2, 0.317 4, 0.319 7, 0.278 5, 1.168 5 μg/♀, respectively, increased by 5.1, 1.1, 0.6, 4.2, and 4.6 times compared with the resistance monitoring data in 2009. Conclusion In Wuhan city, the field monitoring of M. domestica on insecticide resistance level indicated an increasing trend. It was necessary to apply integrated measures and use insecticides scientifically in controlling M. domestica.

Key words: Musca domestica, Insecticide resistance, Trend, Study

摘要： 目的 了解近年来武汉市家蝇抗药性发展趋势，为科学使用杀虫剂提供依据。方法 2009-2016年每年的6-9月，采集武汉市城区农贸市场、垃圾处理场家蝇，实验室饲养1~2代后，用WHO推荐的微量点滴法，测定羽化后3~4日龄雌性成虫的半数致死量（LD₅₀），比较近年来武汉市家蝇抗药性变化情况。结果 2016年武汉市家蝇野外种群对DDVP、甲基吡恶磷、毒死蜱、溴氰菊酯和高效氯氰菊酯的LD₅₀值分别为0.8542、0.3174、0.3197、0.2785、1.1685μg/只，与2009年的抗性监测结果比较，分别增长5.1、1.1、0.6、4.2和4.6倍。结论 武汉市家蝇对常用卫生杀虫剂的抗性呈现增强趋势，在今后灭蝇工作中应采取综合防制措施，科学合理地使用杀虫剂。

关键词: 家蝇, 抗药性, 发展趋势, 研究