Abstract: Objective To investigate the resistance of Musca domestica to commonly used insecticides in Anshun city, Guizhou province, provide guidance in the control of M. domestica. Methods WHO recommended topical application method was used to determine the resistance levels of M. domestica to four insecticides in Xixiu district, development zone and Pingba county. The experimental data were statistically analyzed by SPSS 17.0 software. Results Musca domestica in Anshun city showed different levels of resistance to four commonly used insecticides. Statistics revealed that the resistance to propoxur could not obtain results by using topical application method, which was significantly different from the other three insecticides. The median lethal dose (LD₅₀) of the adult flies against DDVP, beta-cypemethrin, and deltamethrin were 0.526-0.739, 0.135-0.254, and 0.108-0.176 μg, and the resistance ratios were 175.33-246.33, 7.94-14.94, and 15.43-25.14, respectively; that the resistance to DDVP was the highest. Conclusion Musca domestica in Anshun city have developed resistance to the tested insecticides, especially to propoxur and DDVP. Therefore, in practice, insecticides resistance surveillance should be highlighted for wise use of insecticides.

Key words: Musca domestica, Insecticide resistance, Bioassay, Anshun city

摘要： 目的 调查2016年贵州省安顺市家蝇对4种常用杀虫剂的抗药性，为当地家蝇的防控提供理论依据。方法 采用WHO推荐的微量点滴法检测安顺市西秀区、开发区和平坝县家蝇对4种常用杀虫剂的抗药性，利用SPSS 17.0软件进行统计学分析并计算半数致死量（LD₅₀）及其95%CI。结果 安顺市家蝇对4种常用杀虫剂均产生了不同程度的抗药性，其中残杀威已基本失效，无法得出检测结果；对DDVP、高效氯氰菊酯和溴氰菊酯的LD₅₀分别为0.526~0.739、0.135~0.254和0.108~0.176 μg/♀；抗性倍数分别为175.33~246.33、7.94~14.94和15.43~25.14倍，对DDVP的抗性最高，对高效氯氰菊酯的抗性最低。结论 安顺市家蝇对4种常用杀虫剂均产生了抗药性，尤其是对残杀威和DDVP已达极高抗性水平；应加强安顺市病媒生物抗药性监测，科学合理地使用杀虫剂。

关键词: 家蝇, 抗药性, 生物测定, 安顺市