Abstract:

Objective To investigate the distribution and species of ticks and rodents that were involved in the transmission of severe fever with thrombocytopenia syndrome(SFTS). Methods SFTS that occurred at Yaoshang village, Guangfu town, Wuzhong district of Suzhou city was investigated. The free ranging ticks were collected by flagging and the parasitic ticks were collected off the hosts by hand from June 14^th to 18^th in 2016. Small mammals were captured by cage-trapping. The specimens of serum,heart, liver, spleen, lung, and kidney were collected with sterile operations. The severe fever with thrombocytopenia syndrome virus(SFTSV)nucleic acid in the viscera of small animals and in the ticks were detected by real-time PCR. Results A total of 100 ticks were collected, consisting of 4 species, including 37.00% Haemaphysalis sinensis, 29.00% H. flava and 26.00% H. longicornis, separately, which composed the predominant species in Suzhou city. The total number of captured small animals was 17, including 3 species rodents, 1 species Erinaceidae and 1 species insectivora. The results were negative in RNA detection of SFTSV in the specimens of ticks and small animals by real-time PCR. Conclusion The tick vectors and the host animals of SFTSV were found in Suzhou city, but the transmission mechanism between the vectors and the possible hosts need further studies.

Key words: Severe fever with thrombocytopenia syndrome virus, Vector, Investigation

摘要：

目的 调查发热伴血小板减少综合征（SFTS）疫情传播媒介的种群分布及其可疑宿主的感染情况。方法 于2016年6月14－18日，采用人工布旗法和动物体表法，在苏州市吴中区光福镇窑上村SFTS患者住处周围及其经常劳作场所附近环境采集游离蜱和寄生蜱，鼠笼法捕获小兽，取其血清、心、肝、脾、肺、肾和脑标本，利用实时荧光PCR法检测发热伴血小板减少综合征病毒（SFTSV）核酸。结果 共采集蜱4种100只，其中中华血蜱、褐黄血蜱和长角血蜱为优势蜱种，分别占捕获总数的37.00%、29.00%和26.00%。捕获小兽17只，其中啮齿动物3种、食虫动物和猬形动物各1种。蜱及小兽标本SFTSV的核酸经检测均为阴性。结论 江苏省苏州市具有SFTSV媒介蜱及相关宿主动物的分布，但其传播媒介和动物宿主间的传病机制有待进一步研究。

关键词: 发热伴血小板减少综合征病毒, 病媒生物, 调查