Abstract:

Objective Three suspected brucellosis cases were investigated in Suizhou city of Hubei province, and three suspected Brucella strains were identified. Methods Field investigation was applied to finish Brucella epidemiological survey, used Brucella conventional appraisal classification method and the phage cracking test for testing. BCSP31-PCR method (Based on Brucella genus specific gene BCSP31 target gene detection method), AMOS-PCR (based on electrophoresis banding identify B. abortus biovar 1, 2, 4 of Brucella, B. melitensis, B. ovis and B. suis biovar 1) and Real-time PCR, three molecular classification method were compared. Results Three patients were infected by close contact with sick animals and their meat, conventional identification and a variety of polymerase chain reaction (PCR) had the same results, B. melitensis biovar 3. Conclusion Brucella melitensis biovar 3 was identified as the pathogen of the three cases from Suizhou city and cases were mainly caused by direct contact with sick animals and their meat.

Key words: Brucella, Individual survey, Polymerase chain reaction, Identification

摘要：

目的 对湖北省随州市就诊的3例疑似布鲁氏菌病（布病）患者进行个案调查，并对分离的3株疑似布鲁氏菌鉴定分型。方法 应用布病流行病学调查表进行现场调查，采用布鲁氏菌常规鉴定分型方法和噬菌体裂解试验进行检测，并比较BCSP31-PCR法（以布鲁氏菌属特异基因BCSP31为靶基因的检测方法）、AMOS-PCR（根据电泳条带鉴别布鲁氏菌牛种1、2、4型、羊种布鲁氏菌、猪种l型、绵羊附睾种）及实时荧光定量PCR 3种分子分型种型鉴定方法。结果 3例患者均由密切接触病畜及其肉制品感染，常规检测方法鉴定为羊种布鲁氏菌3型，多种PCR方法检测获得同样结果。结论 经常规和PCR鉴定分型研究确定，随州市布病病例分离株为羊种布鲁氏菌3型。

关键词: 布鲁氏菌, 个案调查, 聚合酶链式反应, 鉴定