Abstract:

The artificial medium was prepared for microbial growth, separation, and identification. The commonly used laboratory based was meat infusion agar medium, in which the trypsin digestion agar was superior. It is one of the basic conditions for the separation of Yersinia pestis, playing an important role in pathogen testing, which directly affects the quality and composition of growth, reproduction and isolation of Y. pestis. In this paper, the role of media prepared for Y. pestis, its influencing factors, various aspects of the measures were evaluated in order to constantly improve and enhance the level of quality control medium for plague monitoring.

Key words: Yersinia pestis, Hearst media, Influencing factors

摘要：

培养基是人工配制的适合微生物生长、分离、鉴别的营养物质。国内实验室常用的鼠疫耶尔森菌（鼠疫菌）基础培养基有肉浸液琼脂和胰酶消化液琼脂，其中以胰酶消化液琼脂为优。它是分离鼠疫菌的基本条件之一，在鼠疫病原体检验中发挥着重要作用，其成分和质量直接影响鼠疫菌的分离及保存。通过对制备鼠疫菌基础培养基的作用、影响因素、各环节所采取的措施和对其主要成分的筛选，不断完善和提高培养基的质控水平，并为鼠疫监测工作提供优质的培养基。

关键词: 鼠疫耶尔森菌, 赫氏培养基, 影响因素