Abstract:

Objective In order to analyze the NADH dehydrogenase subunit 1 (ND1) gene of Echinococcus multilocularis from the wild rodents at Alataw pass, China-Kazakhstan. Methods The ND1 genes of E. multilocularis were amplified by PCR. The sequence was determined by Blast and phylogenetic analysis by Mega 6.0. The transmembrane domain (TM), signal peptide and B cell epitope of the ND1 genes of E. multilocularis were predicted and analyzed by DNAStar. Results The positive rate of E. multilocularis was 1.57%. The results showed that the ND1 genes of E. multilocularis had a length of 894 bp, encoding 297 amino acids. E. multilocularis were 100% closely related to the E. multilocularis AB018440 and AB720065. Conclusion The E. multilocularis can infect wild rodents at Alataw pass. The sequence information of E. multilocularis provides a basis for the scientific monitoring.

Key words: Echinococcus multilocularis, Mitochondria, NADH dehydrogenase subunit 1, Gene, Alataw pass

摘要：

目的 分析阿拉山口口岸多房棘球蚴线粒体NADH脱氢酶亚基1（ND1）基因序列。方法 使用ND1基因Cest引物对2014年捕获鼠类进行多房棘球蚴初筛，对阳性样品扩增ND1基因全长序列，利用Blast分析序列同源性、Mega 6.0软件构建分子遗传进化树，使用DNAStar软件分析其跨膜结构、信号肽、B细胞抗原表位等。结果 阿拉山口口岸1.57%的野鼠感染多房棘球蚴，其线粒体 ND1基因全长894 bp，编码297个氨基酸，与日本的多房棘球蚴AB018440和AB720065同源性最高，为100%。结论 阿拉山口口岸地区野鼠存在多房棘球蚴感染，其序列信息为该地区多房棘球蚴的科学监控提供依据。

关键词: 多房棘球蚴, 线粒体, NADH脱氢酶亚基1, 基因, 阿拉山口口岸