Expression profile of aminopeptidase N in different instars of Culex pipiens pallens larvae

doi:10.11853/j.issn.1003.8280.2016.05.007

Chines Journal of Vector Biology and Control ›› 2016, Vol. 27 ›› Issue (5): 450-453.DOI: 10.11853/j.issn.1003.8280.2016.05.007

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Expression profile of aminopeptidase N in different instars of Culex pipiens pallens larvae

GUO Xiu-xia, YANG Pei-pei, CHENG Peng, WANG Hai-fang, LIU Li-juan, ZHANG Chong-xing, WANG Huai-wei, GONG Mao-qing

Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Key Laboratory of Medicine and Health in Shandong Province, Jining 272033, Shandong Province, China

Received:2016-06-16 Online:2016-10-20 Published:2016-10-20
Supported by:
Supported by the National Natural Science Foundation of China (No. 81271877)

淡色库蚊氨肽酶N基因在不同龄期幼虫的表达谱分析

郭秀霞, 杨培培, 程鹏, 王海防, 刘丽娟, 张崇星, 王怀位, 公茂庆

山东省医学科学院, 山东省寄生虫病防治研究所, 山东省医药卫生媒介生物学重点实验室, 山东济宁 272033

通讯作者: 公茂庆,Email:maoqingg@yahoo.com
作者简介:郭秀霞,女,硕士,助理研究员,从事病原生物学研究,Email:guoguoxiua@163.com
基金资助:
国家自然科学基金（81271877）

Abstract

Abstract:

Objective To analyze the expression levels of CpAPN in different instars of Culex pipiens pallens. Methods The APN cDNA sequence was identified and sequenced by quantitative Real-time PCR (qRT-PCR), and the relative expression levels of CpAPN were assessed in different instars of larvae using qRT-PCR assays. Results The full-length of CpAPN was 1 383 bp that encoded a protein of 460 amino acid residues. The homology with Cx. pipiens quinquefasciatus (XM001862270.1) was 100%. The qRT-PCR showed that APN was expressed at all stages of Cx. pipiens pallens, with the highest level in the 4^th instar and the lowest level in the 3^rd instar. There was no significant difference among the 1^st, 2^nd and 4^th instars (F=2.465,P=0.250), but they were all higher than the 3^rd instar significantly (F=49.191, P=0.000), which were 3.5 times, 3.7 times, and 4.0 times of the expression levels to the 3^rd instar, respectively. Conclusion CpAPN showed significantly different expression levels in different instars of Cx. pipiens pallens. The results provided experimental evidences for revealing the function of CpAPN of the mosquitoes.

Key words: Culex pipiens pallens, Aminopeptidase N, Instar, Larvae, Expression profiling

摘要：

目的对淡色库蚊不同龄期幼虫中肠氨肽酶N（Aminopeptidase N，APN）的表达水平进行分析。方法应用反转录-聚合酶链反应（RT-PCR）克隆淡色库蚊APN基因的全长cDNA序列，利用实时荧光定量PCR（qRT-PCR）分析该基因在不同龄期幼虫中肠的表达情况。结果克隆获得淡色库蚊APN基因，该基因全长1 383 bp，编码460个氨基酸，与GenBank收录的致倦库蚊（XM001862270.1）序列的同源性为100%。qRT-PCR表明CpAPN基因在各个龄期均有表达，其中在4龄期的表达量最高，3龄期最低，1、2和4龄期的表达量差异无统计学意义（F=2.465，P=0.250），与3龄期幼虫的差异有统计学意义（F=49.191，P=0.000），分别为其表达量的3.5、3.7和4.0倍。结论 CpAPN基因在淡色库蚊不同龄期幼虫中的表达水平存在差异，为进一步探讨淡色库蚊APN的功能奠定了基础。

关键词: 淡色库蚊, 氨肽酶N, 龄期, 幼虫, 表达谱

CLC Number:

R384.1

GUO Xiu-xia, YANG Pei-pei, CHENG Peng, WANG Hai-fang, LIU Li-juan, ZHANG Chong-xing, WANG Huai-wei, GONG Mao-qing. Expression profile of aminopeptidase N in different instars of Culex pipiens pallens larvae[J]. Chines Journal of Vector Biology and Control, 2016, 27(5): 450-453.

郭秀霞, 杨培培, 程鹏, 王海防, 刘丽娟, 张崇星, 王怀位, 公茂庆. 淡色库蚊氨肽酶N基因在不同龄期幼虫的表达谱分析[J]. 中国媒介生物学及控制杂志, 2016, 27(5): 450-453.

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Expression profile of aminopeptidase N in different instars of Culex pipiens pallens larvae

淡色库蚊氨肽酶N基因在不同龄期幼虫的表达谱分析

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