Abstract:

Objective To monitor the resistance status of Culex pipiens pallens to three kinds of commonly used insecticides in Weifang city for management of insecticide resistance development. Methods Egg rafts of Cx. pipiens pallens were collected from four districts in Weifang, and reared to larvae in insectary. The late-third-to-early-fourth-instar larvae were tested against temephos, deltamethrin and beta-cypermethrin with dipping method to determine the median lethal concentration (LC₅₀). Results The resistance ratios (RRs) to temephos ranged from 7.12 to 11.24 (LC₅₀= 0.003 466-0.005 472 mg/L); to deltamethrin ranged from 1.86 to 3.83 (LC₅₀=0.000 908-0.001 866 mg/L); to beta - cypermethrin were 0.47-1.60 (LC₅₀=0.001 798-0.006 097 mg/L). Conclusion Culex pipiens pallens has developed resistance to temephos, tolerance to deltamethrin, and remained susceptible to beta-cypermethrin. To prevent and control the development of insecticide resistance, insecticides including temephos should be used in rotation or mixture with other pesticides with different modes of action, while avoiding using single active ingredient for long time.

Key words: Culex pipiens pallens, Resistance, Bioassay

摘要：

目的 了解山东省潍坊市城区淡色库蚊对3种常用杀虫剂的抗药性水平，为科学使用杀虫剂提供依据。方法 2015年8月7－10日，在潍坊市4个区采集淡色库蚊卵块，带回实验室饲养；采用浸渍法测定淡色库蚊3龄末4龄初幼虫对杀虫剂的半数致死浓度（LC₅₀）。结果 潍坊市城区淡色库蚊幼虫对双硫磷的LC₅₀ 为0.003 466~0.005 472 mg/L，为敏感品系的7.12~11.24 倍；对溴氰菊酯的LC₅₀ 为0.000 908~0.001 866 mg/L，为敏感品系的1.86~3.83倍；对高效氯氰菊酯的LC₅₀为0.001 798~0.006 097 mg/L，仅为敏感品系的0.47~1.60倍，处于敏感水平。结论 潍坊市城区淡色库蚊对双硫磷为中抗，对溴氰菊酯为低抗或敏感，对高效氯氰菊酯敏感。应避免单一长期使用双硫磷，不同种类杀虫剂交替轮用或复配使用，预防和减缓抗药性的产生。

关键词: 淡色库蚊, 抗药性, 生物学测定