Abstract:

Objective To investigate ticks that harbor emerging tick-borne pathogens and co-infection in lava area of Xunke county of Heilongjiang. Methods A total of 257 live ticks were collected in April to June, 2015. All samples were amplified for specific fragments of 6 emerging tick-borne pathogens by PCR, and further identified through gene sequencing. Results The results showed that 86 positive samples of spotted fever group Rickettsia, infection prevalence was 33.46%; Five positive samples of Bartonella, infection prevalence was 1.94%; 11 positive samples of Anaplasma phagocytophilum, infection prevalence was 4.28%; DNA of Borrelia burgdorferi, Babesia, Ehrlichiosis detection results were all negative. It was also found that 8 co-infection samples existed in Dermacentor silvarum, Ixodes persuleatu, and Haemaphysalis concinna, and the infection prevalence was 3.11%. It was confirmed that co-infection of spotted fever group Rickettsia and Bartonella existed in D. silvarum and coinfection of spotted fever group Rickettsia and An. phagocytophilum existed in I. persuleatus and H. concinna. Conclusion To strengthen the prevention and control of vector ticks in lava area of Xunke county of Heilongjiang is warranted.

Key words: Xunke county of Heilongjiang, Lava area, Live ticks, Emerging tick-borne pathogens, Co-infection

摘要：

目的 调查黑龙江省逊克县火山熔岩地区蜱携带新发蜱传病原体及复合感染情况。方法 2015年4－6月采集活蜱样本257只，利用PCR方法分别扩增6种新发蜱传病原体的特异性核酸片段，通过基因测序进一步鉴定。结果 共检出斑点热群立克次体、巴尔通体和嗜吞噬细胞无形体核酸阳性样本分别为86、5和11例，感染率分别为33.46%、1.94%和4.28%。伯氏疏螺旋体、巴贝西虫和埃立克体的DNA核酸检测结果均为阴性。森林革蜱、嗜群血蜱和全沟硬蜱体内共检出复合感染8例，感染率为3.11%，证实该地区森林革蜱中存在斑点热群立克次体及巴尔通体，嗜群血蜱和全沟硬蜱存在斑点热群立克次体及嗜吞噬细胞无形体复合感染的情况。结论 应加强黑龙江省逊克县火山熔岩地区蜱的防控。

关键词: 黑龙江省逊克县, 火山熔岩地区, 活蜱, 新发蜱传病原体, 复合感染