Abstract:

Objective To investigate the mosquito density and the natural infection of mosquitoes with Japanese encephalitis virus (JEV) in Hanzhuang Town of Weishan county, Lake Taibai of Jining city and Lake Dongping of Taian city, providing scientific basis for controlling mosquitoes and preventing mosquito-borne diseases in the area. Methods Adult mosquito were collected by bed net method and nucleic acids of JEV were detected from mosquito samples by Quantitative Real-time PCR. Results A total of 16 711 mosquitoes were collected from June to September 2015, including 6 genera 17 species. The number of Culex tritaeniorhynchus and Cx. pipiens palllens was 6 610 (39.55%) and 6 465 (38.69%) respectively. They were the predominant in the region. The seasonal fluctuations of all the adult species were consistent, and reaching the density peak in August. JEV was detected from Cx. tritaeniorhynchus, Cx. pipiens palllens and Coquillettidia ochracea (Theobald, 1903). Conclusion The density of mosquito along the south-to-north water diversion project in Shandong is also high and the JEV natural infection exists in a variety of mosquito species. It is the first time that JEV was detected from C. ochracea. All of these findings indicate the region's high JEV infection risk.

Key words: Mosquito, Seasonal fluctuation, Japanese encephalitis virus, Quantitative Real-time PCR

摘要：

目的 在山东省南水北调沿线选择微山韩庄、任城太白湖和泰安东平湖3个地区,调查蚊类密度消长及流行性乙型脑炎(乙脑)病毒自然感染状况,为当地蚊虫控制与乙脑防治工作提供科学依据。方法 2015年6-9月,采用人帐法在上述3个地区采集蚊虫标本,分类计数;将蚊虫标本研磨后提取核酸,采用实时荧光定量PCR法检测乙脑病毒核酸。结果 2015年6-9月在3个监测点共捕获蚊虫6属17种16 711只,以三带喙库蚊(39.55%)和淡色库蚊(38.69%)为优势蚊种;蚊虫密度随季节消长均以8月最高。采用实时荧光定量PCR法,在三带喙库蚊、淡色库蚊、黄色轲蚊中检测到乙脑病毒核酸,批阳性率分别为22.96%、7.58%和16.67%。结论 山东省南水北调沿线蚊虫密度依然较高,且多个蚊种中检测到乙脑病毒核酸,提示该地区存在乙脑病毒感染风险。

关键词: 蚊虫, 季节消长, 流行性乙型脑炎病毒, 实时荧光定量PCR