Abstract:

Objective To study the genotype of Yersinia pestis in Hebei plague foci by variable number of tandem repeat (VNTR). Methods Primers were designed according to the confirmed 14＋12 VNTR, to genotype the 116 Y. pestis DNA of Hebei province. Results All of the strains showed one genetype, but they were different from CO92 and EV. Conclusion There is only one genetype of plague, indicating a genetic stability in Y. pestis in Hebei province.

Key words: Yersinia pestis, Variable number of tandem repeat, Genotyping

摘要：

目的 利用可变数目串联重复序列(VNTR)对河北省鼠疫疫源地菌株进行基因型别研究。方法 根据14+12对VNTR设计引物,对116株鼠疫菌进行分析。结果 116株鼠疫菌针对每对VNTR均有不同的重复数目,但是同一对VNTR对所有菌株的VNTR重复数目相同。与CO92和EV对比,河北省鼠疫疫源地菌株显示出不同的扩增结果。结论 河北省鼠疫疫源地鼠疫菌只有1种不同于其他疫源地的基因型别,为疫情的追踪溯源和鼠疫菌突变监测提供了依据。

关键词: 鼠疫菌, 可变数目串联重复序列, 基因分型