The application of the tandem repeats in the Yersinia pestis genotyping

doi:10.11853/j.issn.1003.4692.2016.01.028

Abstract

Abstract:

The plague is categorized as a Class A infectious disease in China. Plague foci are widely distributed, and strains have different features and varied virulence in China. It is of great significance for deducing the sudden outbreak of plague and terrorist attack detection, with the strains of different foci for genotyping. The choice of tandem repeats loci is the key of the multiple loci vntr analysis (MLVA) classification results. This assay mainly introduces tandem repeats in Yersinia pestis genotyping in application progress, and provides a reference for other workers in genotyping.

Key words: Variable number tandem repeat, Multiple loci vntr analysis, Genetic typing, Yersinia pestis

摘要：

鼠疫为危害人类健康的甲类传染病,由于我国存在着多种类型的鼠疫疫源地,鼠疫菌的特征及致病力不同,因此将不同疫源地的菌株进行基因分型,探讨其亲缘关系,对于鼠疫突发疫情的追溯具有重要意义。多位点可变数目串联重复序列分析方法被认为分型能力较好,但是可变数目串联重复序列(VNTR)位点的选择是分型的关键所在,该文主要介绍VNTR在鼠疫菌基因分型中的应用进展,为基因分型提供参考。

关键词: 可变数目串联重复序列, 多位点可变数目串联重复序列分析, 基因分型, 鼠疫菌

CLC Number:

R254.8

WANG Hai-feng, LIU He-zhi, YANG Shun-lin, ZHOU Song, BAI Xue-wei, YANG Xiao-yan, ZHANG Yi-hui, CHEN Kai-le, LIANG Ying, SHI Xian-ming. The application of the tandem repeats in the Yersinia pestis genotyping[J]. Chines Journal of Vector Biology and Control, 2016, 27(1): 89-91.

王海峰, 刘合智, 杨顺林, 周松, 白雪薇, 杨晓燕, 张懿晖, 陈凯乐, 梁莹, 史献明. 串联重复序列在鼠疫菌基因分型中的应用[J]. 中国媒介生物学及控制杂志, 2016, 27(1): 89-91.

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