Abstract:

Objective To understand the distribution of tick-born diseases in Lishui county, Zhejiang province. Methods The ticks on livestocks and wild animals were collected. The infection of tick-born diseases were detected by PCR amplification with specific primers on rickettsia, Ehrlichia chaffeensis, Anaplasma, Bartonella, Lyme disease, and the barber west protozoa. Results The carrying rate of tick on host animals tested in the present study was 17.84%, while the index of tick carrying was 1.25. The positive rates of rickettsia, Ehrlichia chaffeensis, Anaplasma, Bartonella, Lyme disease, and the barber west protozoa were 13.82%, 35.48%, 44.24%, 2.30%, 9.22%, 9.68%, respectively. Conclusion The carrying rate of tick was high in Lishui county, where more than one pathogen existed. The risk on tick?born infection was high, which should be concerned in terms of public health and well being of humans and livestocks.

Key words: Ticks, Cause of disease carries, Co-infection, Polymerase chain reaction

摘要：

目的 了解丽水市蜱病原体携带情况, 为预防控制蜱传疾病提供科学依据。方法 2012-2013年在放养的家畜和野生动物体表检蜱, 将蜱用75%乙醇消毒及生理盐水清洗反复3次后, 采用QIAamp Tissue Kit核酸提取试剂盒, 按照产品说明书逐步提取DNA。并采用PCR检测立克次体、查菲埃立克体、嗜吞噬细胞无形体、巴尔通体、莱姆病螺旋体、巴贝西原虫等病原体携带情况。结果 宿主动物染蜱率为17.84%, 蜱指数为1.25;蜱感染无形体、查菲埃立克体、立克次体、莱姆病螺旋体、巴尔通体、巴贝西原虫阳性率分别为44.24%、35.48%、13.82%、9.22%、2.30%和9.68%。同时在部分标本中还检出复合感染。结论 丽水市宿主动物染蜱率高, 且蜱存在无形体、查菲埃立克体、立克次体、巴贝西原虫、莱姆病螺旋体、巴尔通体感染, 提示丽水地区发生蜱传疾病流行的风险高, 应引起公共卫生部门重视。

关键词: 蜱, 病原携带, 复合感染, 聚合酶链反应