Abstract:

Objective To understand the strain of serotype, biotype, virulence genes, which cause infantile diarrhea. Methods Bacterial culture, serum agglutination method and PCR were used for serotyping, detection of virulence genes. Results A strain of Yersinia enterocolitica was isolated from the children of feces sample and the short bacillus of gram stain negative was identified under the microscope. Oxidase of the bacteria was negative, katalase was positive, no hydrogen sulfide production, urease test positive, ONPG test positive. The Y. enterocolitica strain is positive in O:3 serotyping test and it belongs to 3 biological types, carrying the virulence genes of ail⁺, ystA⁺, yadA⁺, virF⁺ but no ystB^- . Conclusion The case of infantile diarrhea disease is caused by pathogenic Y. enterocolitica.

Key words: Diarrhea disease, Toxicity-carrying strain, Yersinia enterocolitica

摘要：

目的 了解登封市1株引起幼儿腹泻的小肠结肠炎耶尔森菌的血清型、生物型、毒力基因携带情况。方法 通过细菌培养、血清凝集法及PCR检测技术对分离菌株进行血清分型、毒力基因检测。结果 从幼儿采集的粪便标本中检出小肠结肠炎耶尔森菌,镜下见革兰染色阴性短杆菌。该菌氧化酶阴性,触酶阳性,无硫化氢产生,尿素酶试验阳性,ONPG(邻硝基酚-半乳糖苷酶)试验阳性,血清分型为O:3,生物型为3型。该菌株O:3血清型阳性,生物型3型,毒力基因ail⁺、ystA⁺、ystB^-、yadA⁺、virF⁺。结论 引起幼儿腹泻的病原菌为致病性小肠结肠炎耶尔森菌。

关键词: 腹泻病例, 带毒株, 小肠结肠炎耶尔森菌