Abstract:

Objective To investigate the distribution, species, and seasonality of ticks and rodents and detection of severe fever with thrombocytopenia syndrome virus (SFTSV) in vectors and hosts. Methods In seven counties of Zhejiang province, the free ranging ticks were collected by flagging and the parasitic ticks were collected from the host skin by hand searching on a 3-month basis of 2013. Rodents were captured by squirrelcage method. The specimens of liver and spleen were collected with sterile operations. The SFTSV nucleic acid in the viscera of rodents and in the ticks were detected by fluorescence quantitative RT-PCR. Results A total of 751 ticks were collected, consisting of 2 families, 5 geniuses and 6 species, including 439(58.5%) Haemaphysalis longicornis, which were the predominant species in Zhejiang province. The ticks species distribution varied in different counties in Zhejiang province (χ²=1198.409, P < 0.05). Ticks were active from March to October and the peak was in the second 3-month of the year. The total number of captured rodents was 1078, the rodentia belonged to 13 species in 7 genera in 3 families. The insectivora belonged to 2 species in 2 genera of 2 families. The predominant captures were 355(32.9% ) of Apodemus agrarius and 326(30.2% ) of Eothenomys melanogaster. The distribution of the rodent species also varied in different counties in Zhejiang province (χ²=1623.480, P < 0.05). The results were negative in RNA detection of SFTSV in the specimens of ticks and rodents by RT-PCR. Conclusion The vectors and the host animals of SFTSV were found in Zhejiang province. But the vectors and the possible hosts need further studies.

Key words: Severe fever with thrombocytopenia syndrome bunyavirus, Vector, Host

摘要：

目的 调查浙江省发热伴血小板减少综合征布尼亚病毒(SFTSV)传播媒介蜱和宿主鼠形动物的种群分布、季节消长及其带病毒情况。方法 2013年分4个季度在浙江省7个市(县)采取人工布旗法和动物体表采集法采集游离蜱和寄生蜱;用鼠笼法捕获鼠形动物,取肝、脾标本;用RT-PCR方法检测SFTSV核酸。结果 共捕蜱751只,隶属于1 科6 属10 种,其中长角血蜱为优势蜱种,占58.5%(439/751),不同县(区)蜱种类分布不完全相同(χ²=1198.409,P < 0.05)。3－10月为蜱的活动期,第二季度为蜱的活动高峰期。共捕获鼠形动物1078只,其中啮齿动物3科7属13种,食虫目动物2科2属2种;黑线姬鼠和黑腹绒鼠分别占捕获总数的32.9%(355/1078)和30.2%(326/1078),不同地区的鼠种构成不同(χ²=1623.480,P < 0.05)。蜱及鼠形动物标本新布尼亚病毒核酸检测均为阴性。结论 浙江省具有SFTSV媒介蜱及宿主鼠形动物的分布,但其传播媒介和可能宿主需要进一步调查研究。

关键词: 发热伴血小板减少综合征布尼亚病毒, 媒介, 宿主