Abstract:

Objective To determine the resistance levels of wild populations of Anopheles sinensis to commonly used insecticides in Shanghai and Hunan. Methods The adult mosquito filter paper contact method recommend by WHO was used to determine the resistance levels of An. sinensis to deltamethrin, lambdacyhalothrin, permethrin, BPMC and fenitrothion in Qingpu, Baoshan, Jiading, Jinshan districts, Chongming county of Shanghai and Daoxian county of Hunan. Results Anopheles sinensis in six districts in Shanghai showed different resistance levels to five tested insecticides. High resistance to all five insecticides was detected in Baoshan, as well as to deltamethrin, permethrin and BPMC in Qingpu. Mosquitoes collected from Chongming showed the high resistance to deltamethrin and low resistance to lambdacyhalothrin. Low levels of resistance to lambdacyhalothrin and BPMC was also noted in mosquitoes originated from Jiading. High resistance to deltamethrin, lambdacyhalothrin, BPMC and fenitrothion was encountered in An. sinensis collected from Daoxian county of Hunan. Conclusion Anopheles sinensis has developed resistance to five tested insecticides in Shanghai and Hunan, and cross resistance among pyrethroid, organophosphate and carbamate may exist as revealed by correlation analysis.

Key words: Anopheles sinensis, Resistance monitoring, Chemical insecticide, Effect factor

摘要：

目的 了解上海市以及湖南省永州市道县野外中华按蚊的抗药性水平。方法 用接触筒法测定上海市青浦区、宝山区、嘉定区、金山区、崇明县以及湖南省永州市道县6个地区中华按蚊野外成蚊对溴氰菊酯、高效氯氰菊酯、氯菊酯、仲丁威和杀螟硫磷5种药物的抗性。结果 6个地区的中华按蚊对5种药物均产生了抗性,其中上海市宝山区的中华按蚊对5种药物均达到了高抗性级别;上海市青浦区的中华按蚊对溴氰菊酯、氯菊酯、仲丁威达到了高抗性级别;上海市崇明县的中华按蚊对溴氰菊酯产生了高抗性,对高效氯氰菊酯产生了初步抗性;上海市嘉定区的中华按蚊对高效氯氰菊酯和仲丁威产生了初步抗性;湖南省永州市道县的中华按蚊对溴氰菊酯、高效氯氰菊酯、仲丁威和杀螟硫磷达到了高抗性级别。结论 上海市5个区(县)、湖南省1个县的中华按蚊野外种群均对溴氰菊酯、高效氯氰菊酯、氯菊酯、仲丁威和杀螟硫磷产生了抗性,相关性分析显示拟除虫菊酯类与有机磷类、氨基甲酸酯类之间可能有交互抗性的存在。

关键词: 中华按蚊, 抗药性监测, 化学杀虫剂, 影响因素