Abstract:

Objective To investigate application value of three testing methods on the diagnosis of malaria. Methods There were 102 blood samples of suspicious positive malariafrom 2011 to 2013 in Liaoning. Malaria pathogens were examined using light microscopy (LM), OptiMAL and PCR. Results The positive rate of LM, OptiMAL and PCR were 54.90%, 63.73%, and 70.59%, there was significant difference between LM and PCR. Conclusion In the diagnosis of Plasmodium falciparum, OptiMAL and PCR can be used as complementary methods of the LM.

Key words: Malaria, OptiMAL method, Nested polymerase chain reaction, Light microscopy

摘要：

目的 探讨3种实验室检测方法在疟疾诊断方面的应用价值。方法 分别采用传统镜检法、OptiMAL法及巢式PCR法, 对辽宁省2011－2013年的102份疟疾疑似病例血样进行检测。结果 镜检法、OptiMAL法和PCR法阳性率分别为54.90%、63.73%和70.59%, 三者两两比较, 只有镜检法和PCR法差异有统计学意义(χ²=4.718, P=0.030)。3种方法中, PCR法的敏感性和特异性最好。结论 镜检法结合OptiMAL法和PCR法会提高疟疾病例的诊断水平。

关键词: 疟疾, OptiMAL法, 巢式聚合酶链反应, 镜检法