Abstract:

Objective To investigate the status of rodent, livestock, and poultry carrying Yersinia enterocolitica in plague foci maintained by Citellus dauricus in Ar Horqin Banner, Chifeng, Inner Mongolia, China, and to provide scientific evidence for prevention and control of the disease and study of the plague focus. Methods Villages in the C. dauricus plague foci were chosen as the sampling centers, and the farmland and grassland in outward concentric circles were sequentially included in the sampling area. Feces, tongue roots, throat swabs, and intestinal contents of rodent, livestock, and poultry were separately collected, and different Y. enterocolitica strains were isolated, cultured, identified, and serotyped. PCR analysis was conducted to detect the toxicity genes of Y. enterocolitica. Statistical analysis was performed by chi-square test. Results A total of 3837 samples were collected from 2009 to 2012. Seventeen Y. enterocolitica strains were isolated and the overall detection rate was 0.44%. Of the 16 strains detected in 2011, 13 were from pig feces and 3 from rodent intestines. The pathogenic strain of serotype O:3 and biological type Ⅲ carrying toxicity genes ail, ystA, and rfbC was isolated from pig feces. Conclusion Pigs and rodents in Shaogen, Ar Horqin Banner, carry Y. enterocolitica. Pigs are the major carrier of pathogenic Y. enterocolitica. Further investigation of the distribution pattern of Y. enterocolitica between human and animal hosts in the area is necessary.

Key words: Yersinia enterocolitica, Animal, Toxicity gene

摘要：

目的 了解赤峰市阿鲁科尔沁旗达乌尔黄鼠鼠疫疫源地内鼠类、家畜家禽携带小肠结肠炎耶尔森菌情况,为疾病防治工作和研究鼠疫疫源地提供科学依据。方法 在达乌尔黄鼠鼠疫疫源地内,选择以村庄为中心,向外依次为农田、草原的同心圆样地,分别采集鼠类、家畜家禽粪便、舌根、咽拭子及肠道内容物等样品,进行小肠结肠炎耶尔森菌分离、培养、鉴定、生物血清分型,并用PCR方法进行毒力因子测定。统计学分析采用χ²检验。结果 2009-2012年共检验各类样品3837份,检出小肠结肠炎耶尔森菌17株,总检出率为0.44%。2011年检出的16株小肠结肠炎耶尔森菌中13株源自猪粪,3株来自鼠肠;1株O:3血清型,生物3型,携带ail、ystA、rfbC 毒力基因的致病性菌株检自猪粪。结论 阿鲁科尔沁旗绍根地区的猪、鼠体内携带小肠结肠炎耶尔森菌,猪是致病性小肠结肠炎耶尔森菌的重要携带者。进一步调查分析小肠结肠炎耶尔森菌在该地区人和宿主动物间的分布规律十分必要。

关键词: 小肠结肠炎耶尔森菌, 动物, 毒力基因