Chines Journal of Vector Biology and Control ›› 2014, Vol. 25 ›› Issue (6): 527-533.DOI: 10.11853/j.issn.1003.4692.2014.06.011

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Comparison of knockdown resistance mutations between survival and death populations of Anopheles sinensis after deltamethrin resistance test

SONG Er-ping1, TAN Qi-gan1, GUAN Huai-bin1, SONG Xue-song1, SONG Er-yong1, WU Xiao-chen1, YUAN Liang2,WANG Yu-jiao2, NI Xing2, NI Yang-yang2, ZHANG Liang-liang2, WU Song2   

  1. 1 Feidong Center for Disease Control and Prevention, Hefei 230061, Anhui Province, China;2 Anhui University of Chinese Medicine, Hefei 230038, Anhui Province, China
  • Received:2014-06-12 Online:2014-12-20 Published:2014-12-20
  • Contact: WUSong,Email:jswu167@126.com
  • Supported by:
    Supported by the Natural Science Foundation of Anhui Province of China (No. 10040606Q38), Science Foundation of Hefei Center of Diseases Control and Prevention (No. 2011-03) and Science Foundation for the Excellent Youth Scholars of Education Department of Anhui Province (No. 2009SQRZ119)

中华按蚊溴氰菊酯处理后存活与死亡群体击倒抗性突变型差异比较

宋尔萍1,谈其干1,管怀斌1,宋雪松1,宋尔勇1,吴晓晨1,袁良2,王玉娇2,倪兴2,倪洋洋2,张亮亮2,武松2   

  1. 1 肥东县疾病预防控制中心,安徽 合肥 230061; 2 安徽中医药大学,合肥 230038
  • 通讯作者: 武松,Email: jswu167@126.com
  • 作者简介:宋尔萍,女,主管医师,主要从事疟疾、结核病、艾滋病、麻风病等预防控制工作
  • 基金资助:
    安徽省自然科学基金(10040606Q38); 合肥市疾病预防控制中心科研项目(2011-03); 安徽省优秀青年教师基金(2009SQRZ119)

Abstract: Objective To investigate the effect of knockdown resistance mutations on the deltamethrin resistance of Anopheles sinensis. Methods The deltamethrin resistance of An. sinensis was determined by WHO cones test at six investigation sites in Hefei, China, from 2011 to 2012. Eighty An. sinensis specimens were randomly selected from each of the survival and death populations after the resistance test. DNA was extracted as templates for PCR amplification, and the IIS5-S6 transmembrane segments of voltage?gated sodium channel (VGSC) gene were cloned and sequenced. Chi?square test was used to compare mutation types between the two populations with SPSS software. Results Sequence analysis revealed two types of mutations (TTG to TTT and TTG to TGT) in both groups, with four combinations, i.e., heterozygous mutations TTG(T), TG(T)G(T), and TG(T)T and homozygous mutation (TTT). TGT mutation accounted for 36.9% of the survival population, higher than that of the death population (19.4%) (χ2=17.571, P<0.01), according to the comparison of mutation types (TTG, TTT, and TGT) between the two populations. Conclusion TGT mutation is more associated with deltamethrin resistance than TTT mutation.

Key words: Anopheles sinensis, Knockdown resistance, Voltage-gated sodium channel, Deltamethrin

摘要: 目的 探讨溴氰菊酯抗性中华按蚊群体不同抗性突变类型对蚊虫抗性的影响。方法 对2011及2012年合肥市6个调查点中华按蚊采用WHO接触筒法进行溴氰菊酯敏感性测试,留存抗性测试后存活与死亡中华按蚊标本,两种群体各随机选取80只标本,实验室提取基因组进行钠离子通道蛋白扩增并双向测序,两群体间突变类型比较采用SPSS软件进行χ2检验。结果 测序发现两种群体均存在2种突变类型,即由TTG向TTT及TGT突变。对测序图进行比对发现存在4种突变组合,即TTG(T)杂合、TTT纯合、TG(T)G(T)杂合和TG(T)T杂合。对两种群体TTG、TTT及TGT 3种单倍型构成进行比较,χ2=17.571,P<0.01,存活群体TGT单倍型构成(36.9%)高于死亡群体(19.4%)。结论 TGT抗性突变类型对溴氰菊酯抗性的效应高于TTT突变。

关键词: 中华按蚊, 击倒抗性, 钠离子通道蛋白, 溴氰菊酯

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