Surveillance results of Culex pipiens pallens resistance to commonly used insecticides in Hebei province, China

doi:10.11853/j.issn.1003.4692.2014.06.012

Abstract

Abstract:

Objective To investigate the resistance of Culex pipiens pallens to five commonly used insecticides in Hebei province, China. Methods The susceptibility of Cx. pipiens pallens larvae to commonly used insecticides was determined by WHO larval immersion method. Insecticide resistance was evaluated and compared based on median lethal concentration (LC₅₀) and 95% confidence interval (95%CI). Results The LC₅₀ values of beta-cypermethrin, permethrin, deltamethrin, fenobucarb, and DDVP for 11 field populations of Cx. pipiens pallens were 0.0008-0.1664, 0.0159-0.2280, 0.0006-0.0148, 0.0800-0.6138, and 0.1783-0.5000 μg/ml, respectively. As compared with the previously reported results (1991-1994), Cx. pipiens pallens showed increased resistance to permethrin and slightly decreased resistance to DDVP and deltamethrin. Conclusion The 11 field populations of Cx. pipiens pallens have developed different levels of insecticide resistance. The surveillance of insecticide resistance should be strengthened to provide guidance for the proper application of insecticides.

Key words: Culex pipiens pallens, Insecticide resistance, LC₅₀

摘要：

目的监测河北省淡色库蚊对高效氯氰菊酯、氯菊酯、溴氰菊酯、仲丁威和敌敌畏5种常用杀虫剂的抗性动态。方法用WHO推荐的幼虫浸渍法,测定幼虫对常用杀虫剂的敏感性,获得半数致死浓度(LC₅₀)及95%置信区间(95%CI),比较其抗药性程度。结果 11个淡色库蚊野外种群对高效氯氰菊酯、氯菊酯、溴氰菊酯、仲丁威、敌敌畏的LC₅₀分别为0.0008~0.1664、0.0159~0.2280、0.0006~0.0148、0.0800~0.6138和 0.1783~0.5000 μg/ml。与1991－1994年的抗药性测定结果相比,河北省淡色库蚊对氯菊酯的抗性有增强趋势,对敌敌畏和溴氰菊酯的抗性有略微下降趋势。结论 11个淡色库蚊野外种群对5种杀虫剂均产生了不同程度的抗性,应加强抗药性监测,为科学合理使用杀虫剂提供指导。

关键词: 淡色库蚊, 抗药性, 半数致死浓度

CLC Number:

HAN Xiao-li, HUANG Gang, MA Li-hua, WANG Xi-ming, ZHU Yuan-sheng. Surveillance results of Culex pipiens pallens resistance to commonly used insecticides in Hebei province, China[J]. Chines Journal of Vector Biology and Control, 2014, 25(6): 530-533.

韩晓莉, 黄钢, 马丽华, 王喜明, 朱远生. 河北省淡色库蚊对常用杀虫剂的抗药性监测结果分析[J]. 中国媒介生物学及控制杂志, 2014, 25(6): 530-533.

References

[1] 龚震宇,侯娟,任樟尧,等. 浙江省淡色库蚊和白纹伊蚊对常用化学杀虫剂的抗性调查[J]. 中国媒介生物学及控制杂志,2012,23(5):458-460.
[2] 辛宏业,赵志清,李胜奎. 石家庄市蚊虫3年的抗性实验研究[J]. 医学动物防制,1995,11(1):33-34.
[3] 刘庆芬,张卫成,潘兴昌,等. 衡水市蚊蝇抗药性测定[J]. 医学动物防制,1992,8(1):59.
[4] 刘鉴,周友泉,王金柱,等. 唐山市淡色库蚊幼虫对3种杀虫剂的抗性研究[J]. 医学动物防制,1992,8(1):140-142.
[5] 黄钢,周立强,刘继敏,等. 河北省淡色库蚊对3种杀虫剂的抗性调查[J]. 中国媒介生物学及控制杂志,1995,6(4):313-314.
[6] 褚宏亮,杨维芳,陈东亚,等. 2007－2008年江苏省淡色库蚊和家蝇的抗性研究[J]. 中华卫生杀虫药械,2009,15(5):369-370.
[7] 赵进奎. 洛阳市城区淡色库蚊抗药性现状与预防对策[J]. 医学动物防制,2012,28(4):434-435.
[8] 吴彤宇,张咏梅,张静,等. 天津市2010年蚊蝇抗药性监测[J]. 中国媒介生物学及控制杂志,2012,23(2):122-124.
[9] 白淑萍,孙美玲,张咏梅. 淡色库蚊幼虫对杀虫剂抗性及其防制对策[J]. 医学动物防制,2003,19(12):723-725.
[10] 孟凤霞,靳建超,陈云,等. 我国淡色库蚊/致倦库蚊对常用化学杀虫剂的抗药性[J]. 中国媒介生物学及控制杂志,2011,22(6): 517-520,528.
[11] 刘斯璐,崔峰,燕帅国,等. 中国媒介蚊虫对有机氯类和氨基甲酸酯类杀虫剂的抗性调查[J]. 中国媒介生物学及控制杂志, 2011,22(1):82-85.
[12] 黄清臻. 杀虫剂的科学复配与综合评价[J]. 中华卫生杀虫药械, 2004,10(3):170-173.
[13] 李辛庆,臧继荣,余加席,等. 常用杀虫剂复配及与增效剂混用对蚊虫的灭效研究[J]. 中华卫生杀虫药械,2012,18(1):22-24.
[14] 王新国,王怀位,甄天民,等. 山东省淡色库蚊抗药性及现场治理方法的研究[J]. 中国媒介生物学及控制杂志, 2004, 15(1): 25-26.
[15] 曹勇平,朱礼华,董言德. 具氯菊酯抗性的淡色库蚊幼虫对氯氰菊酯和溴氰菊酯的交互抗性[J]. 南京部队医药,1997(5): 27-28.
[16] 毕富春,王文丽,王秀玲. 家蝇在敌敌畏高负荷环境中的抗性研究[J]. 生态与农村环境学报,1989(2):34-36.