Efficacy of Bacillus thuringiensis serotype H-14 in controlling vector mosquitoes

doi:10.11853/j.issn.1003.4692.2014.06.004

Abstract

Abstract:

Objective To study the activity and efficacy of Bacillus thuringiensis serotype H-14 (B.t. H-14) against vector mosquitoes and the persistence of bacterial liquid preparation. Methods B.t. H-14 with high pathogenicity against mosquitoes was tested in the field sites. The densities of larval and adult mosquitoes were quantified by dippingand human-baited net trapping. The results were compared with the mosquito densities in an untreated control site to determine the field efficacy of B.t. H-14. The persistence of B. thuringiensis in different water bodies was observed in the laboratory. Results The relative population index (RPI) of vector mosquitoes was under 10 during peak periods, which indicated that B.t. H-14 lowered the populations of vector mosquitoes in natural habitats. The mosquito densities in the treated site remained at a relatively low level, with a larval density less than 50/dip and an adult density less than 20/hour. However, the density of mosquitoes in the untreated control site changed considerably with seasons, with a larval density more than 100/dip and an adult density up to 188/hour. In the laboratory, the activity of B.t. H-14 lasted 30 d. The larvicidal activity of B.t. H-14 was optimal in pure water; in polluted water, however, its activity reached the peakin one weekand then decreased gradually. Conclusion In the natural environment, B.t. H-14 can be used to control vector mosquitoes, and the concentration of bacterial liquid applied and the number of applications can be determined according to the mosquito population densities.

Key words: Bacillus thuringiensis, Vector mosquito, Biological control, Culex pipiens pallens

摘要：

目的研究苏云金杆菌对媒介蚊虫的防治效果以及菌液在室内的持效性。方法选择对媒介蚊虫具有高致病力的血清型苏云金杆菌缓释悬浮剂(B.t. H-14)防治试验区蚊虫,分别利用勺捕法和人帐诱法调查蚊幼虫和成蚊密度,与对照区蚊虫密度比较,研究B.t. H-14的现场防治效果,并在实验室观察苏云金杆菌在不同水体内的持效性。结果利用苏云金杆菌防治媒介蚊虫,在媒介蚊虫高峰季节,相关密度指数(RPI)<10,说明苏云金杆菌可以控制自然水体内媒介蚊虫的种群数量。试验区蚊虫密度维持在较低水平,幼虫密度<50条/勺,成蚊密度<20只/h,而对照区蚊虫密度随季节变化明显,幼虫密度>100条/勺,成蚊密度最高可达188只/h。在实验室内,苏云金杆菌持效期可达30 d,其中,在清水中苏云金杆菌的防治效果最好,污染水体苏云金杆菌对蚊幼虫的杀灭率在1周后达到最大值,以后逐渐降低。结论在自然环境中可利用苏云金杆菌防治媒介蚊虫,并根据蚊虫密度,施用不同浓度的菌液和确定施药次数。

关键词: 苏云金杆菌, 媒介蚊虫, 生物防治, 淡色库蚊

CLC Number:

R384.1
S476

WEI Ji-bo, LI Gui-ling, MENG Qing-dong, LIU Li-juan, DAI Yu-hua, WANG Huai-wei. Efficacy of Bacillus thuringiensis serotype H-14 in controlling vector mosquitoes[J]. Chines Journal of Vector Biology and Control, 2014, 25(6): 502-505.

魏继波, 李桂玲, 孟庆东, 刘丽娟, 代玉华, 王怀位. 苏云金杆菌对媒介蚊虫的防治研究[J]. 中国媒介生物学及控制杂志, 2014, 25(6): 502-505.

References

[1] 李士根,王兆杰,全芯,等. 山东省淡色库蚊对化学杀虫剂的抗性及防治对策[J]. 中国媒介生物学及控制杂志,2010,21(5):434-436.
[2] 孟凤霞,靳建超,陈云,等. 我国淡色库蚊/致倦库蚊对常用化学杀虫剂的抗药性[J]. 中国媒介生物学及控制杂志,2011,22(6):517-520,528.
[3] Kumar PM, Murugan K, Kovendan K, et al. Mosquito larvicidal and pupicidal efficacy of Solanum xanthocarpum (Family: Solanaceae) leaf extract and bacterial insecticide, Bacillus thuringiensis, against Culex quinquefasciatus Say (Diptera: Culicidae)[J]. Parasitol Res,2012,110(6):2541-2550.
[4] 徐健,赵松,刘琴. 苏云金杆菌以色列亚种生物杀蚊幼颗粒剂及其作用特性[J]. 中国血吸虫病防治杂志,2013,25(1):52-55.
[5] Darsi S, Prakash GD,Udayasuriyan V. Cloning and characterization of truncated cry1Ab gene from a new indigenous isolate of Bacillus thuringiensis[J]. Biotechnol Lett,2010,32(9):1311-1315.
[6] da Costa JR, Rossi JR, Marucci SC, et al. Toxic activity of Bacillus thuringiensis isolates to Aedes aegypti (L.) (Diptera: Culicidae) larvae[J]. Neotrop Entomol,2010,39(5):757-766.
[7] Balaraman K. Occurrence and diversity of mosquitocidal strains of Bacillus thuringiensis[J]. J Vector Borne Dis,2005,42(3):81-86.
[8] 陆宝麟. 蚊虫综合治理[M]. 北京:科学出版社,1999:118-123.
[9] Lee YW, Zairi J, Yap HH. Integration of Bacillus thuringiensis H-14 formulations and pyriproxyfen for the control of larvae of Aedes aegypti and Aedes albopictus[J]. J Am Mosq Control Assoc, 2005,21(1):84-89.
[10] 陈登宏,房天职,杨新史,等. 苏云金杆菌H-14对蚊幼虫的毒力测定及现场防制效果[J]. 医学动物防制,1991,7(3):222-225.
[11] 王美秀,王淑京,杨晓杰,等. 两种剂型苏云金杆菌血清14型制剂对埃及伊蚊幼虫的毒杀效果试验[J]. 中国媒介生物学及控制杂志,1996,7(1):70.
[12] 消毒杀虫灭鼠手册编写组. 消毒杀虫灭鼠手册[M]. 北京:人民卫生出版社,1980:218-219,319-320.
[13] Loke SR,Andy-Tan WA, Benjamin S, et al. Susceptibility of field-collected Aedes aegypti (L.) (Diptera: Culicidae) to Bacillus thuringiensis israelensis and temephos[J]. Trop Biomed,2010,27(3):493-503.
[14] Paris M, David JP, Despres L. Fitness costs of resistance to Bti toxins in the dengue vector Aedes aegypti[J]. Ecotoxicology,2011,20(6):1184-1194.
[15] 代玉华,黄晓丹,刘丽娟,等. 济宁地区淡色库蚊幼虫对苏云金杆菌的抗性水平研究[J]. 寄生虫与医学昆虫学报,2011,18(2):99-102.
[16] 鲁睿. 苏云金杆菌和麦蛾柔茧蜂对印度谷螟的联合控制作用研究[D]. 武汉:华中农业大学,2008.
[17] Ketseoglou I, Bouwer G. The susceptibility of five African Anopheles species to Anabaena PCC 7120 expressing Bacillus thuringiensis subsp. israelensis mosquitocidal cry genes[J]. Parasit Vectors,2012,5:220. doi:10.1186/1756-3305-5-220.
[18] 代玉华,程鹏,刘丽娟,等. 苏云金杆菌对不同龄期淡色库蚊幼虫的灭杀作用[J]. 中华卫生杀虫药械,2011,17(1):29-30.
[19] 易建荣,林立丰. 苏云金杆菌悬浮剂杀灭白纹伊蚊不同龄期幼虫实验及其影响因素分析[J]. 中华卫生杀虫药械,2003,9(2):15-18.
[20] 苏善强,甘保吉,陈惟吾,等. 苏云金杆菌H-14块状缓释剂控制家庭积水容器中埃及伊蚊幼虫的效果观察[J]. 中国媒介生物学及控制杂志,1995,6(6):404-407.
[21] 黄丽华,冯向阳,耿文奎. 氯氰菊酯与苏云金杆菌混合制剂和苏云金杆菌制剂对致乏库蚊幼虫的毒效比较[J]. 医学动物防制,2001,17(10):532-533.
[22] Armengol G, Hernandez J, Velez JG, et al. Long-lasting effects of a Bacillus thuringiensis serovar israelensis experimental tablet formulation for Aedes aegypti (Diptera: Culicidae) control[J]. J Econ Entomol,2006,99(5):1590-1595.
[23] Jacups SP, Rapley LP, Johnson PH,et al. Bacillus thuringiensis var. israelensis misting for control of Aedes in cryptic ground containers in north Queensland,Australia[J]. Am J Trop Med Hyg, 2013,88(3):490-496.