Abstract: Objective　To analyze the single nucleotide polymorphisms (SNPs) in HLA-B gene and HLA-DQ gene in hemorrhagic fever with renal syndrome (HFRS) patients and healthy people and to study the relationship between Hantavirus infection and genetic characteristics of humans. Methods　Five SNPs in HLA-B gene and HLA-DQ gene were amplified using allele-specific primer PCR (ASP-PCR). The association between SNPs and Hantavirus infection was investigated by case-control study. Results　According to the result of the HFRS antibody diagnostic assay, 24 Hantavirus-positive whole blood samples were included. Negative samples were collected from 51 healthy people. The frequencies of CC genotype of rs34933313 in the case group and control group were 4.17% and 29.41%, respectively; the frequencies of GG genotype were 12.50% and 15.69%, respectively; the frequencies of CG genotype were 83.33% and 54.90%, respectively. The frequencies of CC and GG in the case group were significantly lower than those in the control group, and the frequency of CG genotype in the case group was significantly higher than that in the control group; significant difference in the genotype of rs34933313 was found between the two groups (χ2＝7.050, P＝0.029). No significant difference was found at any of the other four sites. Conclusion　SNP at rs34933313 might be associated with Hantavirus infection and may increase the risk of Hantavirus infection in humans. HLA-B gene is probably related to Hantavirus infection in humans.

Key words: Hemorrhagic fever with renal syndrome, Hantavirus, Single nucleotide polymorphism

摘要： 目的　分析肾综合征出血热(HFRS)患者和健康人群HLA?B及HLA?DQ基因区域的单核苷酸多态性(SNP)位点,探讨人感染汉坦病毒与其遗传特性间的关联性。方法　采用等位基因特异性引物扩增(ASP?PCR)技术对HLA?B及HLA?DQ区域上的5个SNP位点进行扩增,利用病例对照研究探讨SNP与人群汉坦病毒感染状态之间的关系。结果　筛选HFRS患者阳性标本24份,同时采集与病例相匹配的健康人群标本51份。rs34933313位点患者组和对照组的CC基因型频率分别为4.17%和29.41%,GG基因型为12.50%和15.69%,CG基因型为83.33%和54.90%,CC、GG基因型频率患者组低于对照组,CG基因型频率患者组高于对照组,且二者之间差异有统计学意义(χ2＝7.050,P＝0.029)。其他4个位点两组间的差异均无统计学意义。结论　rs34933313位点的基因多态性可能与人群汉坦病毒感染有关,可能会增加人群感染该病毒的风险。HLA?B基因可能与人群感染汉坦病毒存在相关性。

关键词: 肾综合征出血热, 汉坦病毒, 单核苷酸多态性