Abstract:

Objective To determine the chemical property and attractive activity of volatiles from Blattella germanica. Methods Volatiles from B. germanica were collected by headspace SPME method in purge flow. GC?MS was used to identify the main components in SPME extracts. GC?EAD was used to identify electrophysiologically active components. Active components were used for trapping test. Results Analysis of the extracts after SPME revealed that there were at least 19 components in the volatiles, with 13 volatile C2-C5 carboxylic acids and alcohols as main components. GC?EAD test with standard chemicals showed that only 6 volatile carboxylic acids elicited electroantennogram responses. Field trapping test showed that the mixture of 6 volatile carboxylic acids were effective in attracting B. germanica. Conclusion Attractive volatiles emitted by B. germanica play a role in colony aggregation.

Key words: Blattella germanica, Volatile, Electroantennogram response

摘要：

目的 查明德国小蠊自身挥发物的成分及其引诱活性。方法 使用顶空吹扫固相微萃取(HS?SPME)提取德国小蠊挥发物, 并通过气相-质谱(GC?MS)联用分析SPME提取物中的主要成分, 使用气相-触角电位(GC?EAD)来分析电生理活性的主要组分, 并使用有活性的组分进行诱捕实验。结果 发现德国小蠊挥发物中包含至少19个组分, 其中含量较高的13个组分分别是一些C2～C5的挥发性醇和酸。用标准化合物样品所做的GC?EAD分析结果表明, 仅6种挥发羧酸对德国小蠊具有明显的触角电位反应。现场诱捕实验证实, 酸类物质混配后对德国小蠊具有一定的引诱效果。结论 德国小蠊释放有活性的挥发物并可起到一定引诱作用, 引起群体聚集。

关键词: 德国小蠊, 挥发物, 触角电位反应