A survey of contamination with Salmonella in broiler chickens in Hefei, China

doi:10.11853/j.issn.1003.4692.2014.01.012

Abstract

Abstract: Objective To investigate the contamination, serotypes and antibiotic resistance profiles of Salmonella, and its genotypes determined by pulsed-field gel electrophoresis (PFGE) in broiler chickens during breeding and slaughtering processing in Hefei, China, and to provide scientific baseline data for the food safety and a scientific basis for strengthening the public health security in Hefei. Methods From July to September 2010, the anal swab method and carcass rinsing method were used to collect samples from 45 live broilers and 45 broiler carcasses in 10 broiler farms and 14 slaughter houses in Hefei. According to GB/T 4789.4-2010, Salmonella was isolated and identified and then subjected to serological analysis; PCR was used for identification of suspected Salmonella isolates. The disk diffusion method recommended by the Clinical and Laboratory Standards Institute was used for testing the drug sensitivity of the finally identified Salmonella strains. PFGE was used for genotyping of these Salmonella strains. Results No Salmonella strain was detected in the 45 live broiler samples; 12 Salmonella strains were detected in the 45 broiler carcass samples, with a detection rate of 26.7%. The PCR testing indicated that all the 12 Salmonella strains carried genes invA and hilA. The serological analysis showed that among the 12 Salmonella strains, 9 were S. indiana, 2 were S. typhimurium, and 1 was S. enteritidis. Ten of the 12 Salmonella strains were resistant to the 15 antibiotics used in the experiment; also, 10 strains developed multi?drug resistance. Seven types of PFGE patterns were identified among the 12 Salmonella strains, and 5 types among the 9 strains of S. indiana. The genotyping showed that the Salmonella strains of the same serotype were almost in the same large cluster. The Salmonella strains of the same PFGE pattern had similar antibiotic resistance profiles. Conclusion In Hefei, the Salmonella detection rate among broiler carcasses is high; Salmonella strains are diverse in serotypes, drug sensitivity profiles, and genotypes; many of them are resistant to multiple antibiotics, and the strains of the same PFGE pattern have similar antibiotic resistance profiles.

Key words: Broiler, Salmonella, Serotype, Antibacterial susceptibility, Genotype

摘要： 目的了解合肥市养殖屠宰环节中肉鸡沙门菌的带菌率、血清型分布、抗生素耐药谱分布以及脉冲场凝胶电泳(PFGE)分子分型情况,为合肥市食品安全提供科学的基础数据,为进一步加强合肥市公共卫生安全提供科学依据。方法 2010年7－9月对合肥市10家肉鸡养殖场和14家屠宰户分别采用肛拭法和胴体漂洗法采集45份肉鸡活体和45份肉鸡胴体标本,根据GB/T 4789.4－2010对沙门菌进行分离、鉴定以及血清学分析;并采用PCR方法进行复核鉴定;对最终确定的阳性菌株使用临床实验室标准化协会(CLSI)推荐的纸片法进行药敏试验;同时采用PFGE方法确定其分子分型。结果在45份肉鸡活体样本中未检出沙门菌;45份肉鸡胴体样本中检出12株沙门菌,检出率为26.7%,经PCR复核检测,所分离的12株沙门菌对invA和hilA基因的携带率均为100%;12株沙门菌分为3种血清型,其中9株为印第安纳沙门菌,2株为鼠伤寒沙门菌,1株为肠炎沙门菌;12株沙门菌对实验中所采用的15种抗生素总耐药以及多重耐药菌株数均为10株;12株沙门菌共分出7种PFGE带型,9株印第安纳沙门菌共分离出5种不同的PFGE带型;同一血清型的沙门菌分子分型结果,基本位于同一大簇中;相同PFGE带型的菌株,耐药谱非常接近。结论合肥市肉鸡胴体沙门菌检出率高,沙门菌的血清型、药敏性以及基因型具有多样性特征,耐药现象严重,相同PFGE带型的菌株,耐药谱非常接近。

关键词: 肉鸡, 沙门菌, 血清型, 耐药性, 分子分型

CLC Number:

YUE Feng, WANG Hai, DU Guo-qiang, TANG Ren-shu, CUI Zhi-gang, TAO Fang-biao. A survey of contamination with Salmonella in broiler chickens in Hefei, China[J]. Chines Journal of Vector Biology and Control, 2014, 25(1): 43-46.

岳峰,王海,杜国强,汤仁树,崔志刚,陶芳标. 合肥市肉鸡沙门菌携带情况调查[J]. 中国媒介生物学及控制杂志, 2014, 25(1): 43-46.

References

[1] Lauderdale TL,Aarestrup FM,Chen PC, et al. Multidrug resistance among different serotypes of clinical Salmonella isolates in Taiwan[J]. Diagn Microbiol Infect Dis,2006,55(2):149-155.
[2] Olsen SJ, Bishop R, Brenner FW,et al. The changing epidemiology of Salmonella: trends in serotypes isolated from humans in the United States,1987-1997[J]. J Infect Dis,2001,183:753-761.
[3] Yang BW, Qu D, Zhang XL, et al. Prevalence and characterization of Salmonella serovars in retail meats of marketplace in Shanxi, China[J]. Int J Food Microbiol,2010,141:63-72.
[4] Callaway TR, Edrington TS, Anderson RC, et al. Gastrointestinal microbial ecology and the safety of our food supply as related to Salmonella[J]. J Anim Sci,2008,86:e163-172.
[5] 中华人民共和国卫生部. GB 4789.4－2010 食品安全国家标准食品微生物学检验沙门菌检验[S]. 北京:中国标准出版社,2010.
[6] Ranh K,de Crandis SA, Clarke RC, et al. Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella[J]. Mol Cell Probes,1992,6(4):271-279.
[7] Guo X, Chen JR, Beuchat LR, et al. PCR Detection of Salmonella enterica serotype Montevideo in and on raw tomatoes using primes derived from hilA[J]. Appl Environ Mocrobiol,2000,66(12): 5248-5252.
[8] Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard[M]. 2nd ed. Wayne,PA:CLSI,2009:19-38.
[9] Sandt CH,Krouse DA, Cook CR, et al. The key role of pulsed-field gel electrophoresis in investigation of a large multi serotype and multistate food-borne outbreak of Salmonella infections centered in Pennsylvania[J]. J Clin Microbiol,2006,44(9):3208-3212.
[10] 王军,郑增忍,王晶钰. 动物源性食品中沙门菌的风险评估[J]. 中国动物检疫,2007,24(4):3-25.
[11] 王燕梅,乔昕,符晓梅,等. 2010年江苏省肉鸡沙门菌污染专项监测分析[J]. 中国食品卫生杂志,2012,24(2):170-172.
[12] 李薇薇,白莉,张秀丽,等. 中国四省肉鸡生产加工环节沙门菌的污染及耐药谱分布状况[J]. 中华预防医学杂志,2013,47(5): 435-438.
[13] European Food Safety Authority. Report of the task force on zoonoses data collection on the analysis of the baseline survey on the prevalence of Salmonella in broiler flocks of Gallus gallus, in the EU, 2005-2006, Part A: Salmonella prevalence estimates[J]. The EFSA J,2007,98:1-85.
[14] 杨保伟,盛敏,席美丽,等. 沙门菌抗生素抗性机理研究进展[J]. 微生物学通报,2008,35(9):1479-1484.
[15] 杨保伟,申进玲,席美丽,等. 2007－2008年西安地区鸡肉源沙门菌相关特性分析[J]. 食品科学,2011,32(19):130-136.
[16] Barrett TJ,Gerner-Smidt P,Swaminathan B. Interpretation of pulsed field gel electrophoresis patterns in foodborne disease investigations and surveillance[J]. Foodborne Pathog Dis,2006,3(1):20-31.
[17] 王丽丽,徐建国. 脉冲场凝胶电泳技术(PFGE)在分子分型中的应用现状[J]. 疾病监测,2006,21(5):276-279.
[18] Tsen HY, Lin JS, Hu HH, et al. Use of pulsed field gel electrophoresis as an epidemiological tool for analysis of sporadic associated strains of Salmonella typhi isolated in Taiwan[J]. J Appl Microbiol,1999,86(5):761-768.
[19] Tenover FC, Arbeit RD, Goering RV, et al. Interpreting Chromosomal DNA restriction patterns produced by pulsed?field gel electrophoresis: criteria for bacterial strain typing[J]. J Clin Microbiol,1995,33(9):2233-2239.