Investigation of Bartonella infection in rodents in Zhejiang province, China

doi:10.11853/j.issn.1003.4692.2014.01.007

Chines Journal of Vector Biology and Control ›› 2014, Vol. 25 ›› Issue (1): 24-27.DOI: 10.11853/j.issn.1003.4692.2014.01.007

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Investigation of Bartonella infection in rodents in Zhejiang province, China

LING Feng^1,2, DING Feng³, GONG Zhen-yu¹, CHEN En-fu¹, HOU Juan¹, MIAO Zi-ping¹,SHI Xu-guang¹, QIN Shu-wen¹, LI Dong-mei², LIU Qi-yong²

1 Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China;
2 State Key Laboratoryfor Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, ChineseCenter for Disease Control and Prevention, Beijing 102206, China;
3 Haining Center for Disease Control and Prevention

Received:2013-06-24 Online:2014-02-20 Published:2014-02-20
Contact: LIU Qi-yong, Email: liuqiyong@icdc.cn; GONG Zhen-yu, Email: zhygong@cdc.zj.cn
Supported by:
Supported by the National Science and Technology Major Project for Infectious Diseases in China (No. 2012ZX10004-219), Key Program from Science Technology Department of Zhejiang (No. 2012C13016-2), and the Medical Research Program of Zhejiang (No. 2009A045)

浙江省啮齿动物巴尔通体感染状况调查

凌锋¹,²,丁丰³,龚震宇¹,陈恩富¹,侯娟¹,缪梓萍¹,施旭光¹,秦淑文¹,栗冬梅²,刘起勇²

1 浙江省疾病预防控制中心传染病预防控制所,浙江杭州 310051;
2 中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206;
3 海宁市疾病预防控制中心

通讯作者: 刘起勇, Email: liuqiyong@icdc.cn; 龚震宇, Email: zhygong@cdc.zj.cn
作者简介:凌锋, 男, 主管医师, 主要从事病媒生物及病媒生物性传染病预防控制工作。Email: fengl@cdc.zj.cn
基金资助:
国家传染病科技重大专项(2012ZX10004-219); 浙江省科技厅重大专项(2012C13016-2); 浙江省医药卫生科技项目(2009A045)

Abstract

Abstract: Objective To investigate the Bartonella infection in rodents in Zhejiang province, China and to provide baseline data for the control and prevention of Bartonella infection in humans. Methods Rodents were captured by night trapping in different areas and seasons in Zhejiang province, and their liver and spleen were aseptically collected to detect Bartonella by PCR. Some positive amplicons were sequenced and the nucleotide sequences tested were submitted to GenBank. Similarity comparison and sequence analysis were then performed to identify the Bartonella species. Results The specific DNA fragments of Bartonella were detected in Apodemus agrarius (5.76%, 17/295), Rattus norvegicus (2.86%, 1/35), Mus musculus (10.00%, 1/10), Microtus fortis (5.26%, 1/19), Niviventer confucianus (3.91%, 7/179), and Rattus losea (3.03%, 1/33). Furthermore, Bartonella DNA fragments were detected from M. musculus for the first time in Zhejiang. The positive rates were 2.63% (3/114), 10.84% (9/83), 2.88% (3/104), 11.11% (12/108), 0% (0/46), and 0.57% (1/175) in Daishan, Haining, Jiande, Lishui, Xianju, and Longyou, respectively. The positive rates were 3.39% (4/118), 6.08% (9/148), 2.95% (7/237), and 6.30% (8/127) in the four seasons, respectively. The average positive rate was 4.44% (28/630). Phylogenetic analysis suggested that the detected Bartonella had the closest genetic relationship with Bartonella henselae. Conclusion The prevalence of Bartonella infection in rodents in Zhejiang province has posed a risk of transmission to humans, and corresponding control measures should be taken into consideration.

Key words: Rodent, Bartonella, Investigation

摘要： 目的调查浙江省啮齿动物巴尔通体携带状况,为巴尔通体人群感染的预防控制提供科学依据。方法用夹夜法在浙江省不同地区、不同季节捕获啮齿动物,无菌操作取啮齿动物的肝和脾,用聚合酶链反应(PCR)检测巴尔通体,然后对部分阳性产物测序,将所测核酸序列提交到GenBank,做相似性比较及序列分析。结果从黑线姬鼠、褐家鼠、小家鼠、东方田鼠、北社鼠和黄毛鼠中检测到巴尔通体特异DNA片段,阳性率分别为5.76%(17/295)、2.86%(1/35)、10.00%(1/10)、5.26%(1/19)、3.91%(7/179)和3.03%(1/33),浙江省首次从小家鼠中检测到巴尔通体DNA片段;岱山、海宁、建德、丽水、仙居、龙游地区的阳性率分别为2.63%(3/114)、10.84%(9/83)、2.88%(3/104)、11.11%(12/108)、0(0/46)和0.57%(1/175);4个季度阳性率分别为3.39%(4/118)、6.08%(9/148)、2.95%(7/237)、6.30%(8/127),平均阳性率为4.44%(28/630);遗传进化分析提示检测到的巴尔通体与Bartonella henselae遗传关系最接近。结论浙江省啮齿动物中广泛存在巴尔通体感染,存在对人群致病的风险,应采取措施加以控制。

关键词: 啮齿动物, 巴尔通体, 调查

CLC Number:

LING Feng, DING Feng, GONG Zhen-yu, CHEN En-fu, HOU Juan, MIAO Zi-ping,SHI Xu-guang, QIN Shu-wen, LI Dong-mei, LIU Qi-yong. Investigation of Bartonella infection in rodents in Zhejiang province, China[J]. Chines Journal of Vector Biology and Control, 2014, 25(1): 24-27.

凌锋,,丁丰,龚震宇,陈恩富,侯娟,缪梓萍,施旭光,秦淑文,栗冬梅,刘起勇. 浙江省啮齿动物巴尔通体感染状况调查[J]. 中国媒介生物学及控制杂志, 2014, 25(1): 24-27.

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Investigation of Bartonella infection in rodents in Zhejiang province, China

浙江省啮齿动物巴尔通体感染状况调查

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