Abstract:

Objective To compare the quality between the glass specimens of acaroid mites prepared with three mounting media. Methods Polyethylene lactic acid phenol medium, Berlese medium, and Hoyer's medium were prepared according to formulae, and their physical and chemical properties were compared. Sixty glass specimens of acaroid mites were prepared with each medium and were observed under a optical microscope after being oven-dried. The obtained data were analyzed by chi-square test. Results Polyethylene lactic acid phenol medium was light yellow liquid, with a density of 0.99 g/ml. Berlese medium was reddish brown, with a density of 1.12 g/ml. Hoyer's medium was slightly lighter than Berlese medium, with a density of 1.37 g/ml. The optical microscopy showed that the glass specimens of acaroid mites prepared with Hoyer's medium had high transparency and stretched mite bodies, with little yellowing and blackening at both sides of mite bodies; the specimens prepared with the other two media had relatively low quality. The chi-square test revealed significant differences between the three media (χ²=15.54, P<0.01), and the specimens prepared with Hoyer's medium had significantly higher quality than that prepared with polyethylene lactic acid phenol medium (χ²=7.50, P<0.01) and that prepared with Berlese medium (χ²=15.70, P<0.01). Conclusion Glass specimens of acaroid mites prepared with Hoyer's medium have the best quality under a optical microscope, providing a reference for the preparation of high-quality glass specimens of acaroid mites.

Key words: Medium, Mite specimen, Optical microscope, Hoyer’s medium

摘要：

目的 比较3种封固剂制作螨标本玻片的效果。方法 按配方配制多乙烯乳酸酚、贝氏和霍氏3种封固剂,比较它们的理化性质,并分别制作粉螨玻片标本各60张,烘干后在光学显微镜下观察制片效果,χ²检验进行统计学分析。结果 多乙烯乳酸酚封固剂为淡黄色的透明液体,密度为0.99 g/ml;贝氏封固剂红棕色,密度为1.12 g/ml;霍氏封固剂颜色较贝氏稍淡,密度为1.37 g/ml。光学显微镜下,霍氏封固剂制作的粉螨标本透明度好,螨体舒展,很少出现躯体两侧发黄发焦的现象;多乙烯乳酸酚和贝氏封固剂效果欠佳。经χ²检验,三者之间差异有统计学意义(χ²=15.54,P<0.01),且霍氏封固剂的制片效果优于多乙烯乳酸酚(χ²=7.50,P<0.01)和贝氏(χ²=15.70,P<0.01)封固剂。结论 霍氏封固剂制备粉螨标本玻片观察效果较好,可为高质量的螨标本制备提供参考。

关键词: 封固剂, 螨标本, 光学显微镜, 霍氏封固剂