Molecular evolution analysis of COⅠ gene in five species of Culex

Chines Journal of Vector Biology and Control ›› 2013, Vol. 24 ›› Issue (1): 28-30.

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Molecular evolution analysis of COⅠ gene in five species of Culex

LAN Ce-jie¹, SHEN Yuan¹, LU Liang², MA Guang-yuan³, WU Jia-lin³

1. Disinfection and Vector Control Section, Wuxi Center for Disease Control and Prevention, Wuxi 214023, Jiangsu Province, China;
2. State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention;
3. Molecular Biology Laboratory, Wuxi Center for Disease Control and Prevention

Received:2012-08-26 Online:2013-02-20 Published:2013-02-20
Supported by:
Supported by the Preventive Medicine Projects of Health Department of Jiangsu(No. Y201011) and Health Systems Research Projects of Wuxi(No. XM1001)

5种库蚊的COⅠ基因分子进化分析

兰策介¹, 沈元¹, 鲁亮², 马广源³, 吴家林³

1. 无锡市疾病预防控制中心消毒与媒介生物防制科,江苏无锡214023;
2. 中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室;
3. 无锡市疾病预防控制中心分子生物学实验室

基金资助:
江苏省卫生厅预防医学课题(Y201011);无锡市卫生系统科研项目(XM1001)

Abstract

Abstract: Objective To investigate the relationship between cytochrome oxidase subunitⅠ(COⅠ) gene traits and molecular evolution of Culex in Wuxi, China. Methods COⅠ genes of Cx. pipiens pallens, Cx. tritaeniorhynchus, Cx. bitaeniorhynchus, Cx. mimulus, and Cx. fuscanus were amplified, sequenced, and blasted in GenBank. Gene traits, transversion rates, and divergence times were analyzed. Molecular phylogenetic trees were generated using Mega 4.1. Results COⅠgene of Cx. mimulus was sequenced for the first time. The COⅠ genes studied were 692 bp to 717 bp. GC contents were from 29.91% to 31.42%. Transversion rates were from 5.610% to 8.555%. Divergence times were from 2.44×10⁶a to 3.72×10⁶a. Each species of Culex showed high support for a monophyletic group (BPP≥98). Conclusion COⅠ gene has species specificity, and it can be used for the identification of the five species of Culex.

Key words: Culex, COⅠ gene, Molecular evolution

摘要： 目的了解无锡市库蚊细胞色素氧化酶亚基Ⅰ(COⅠ)基因特征和蚊虫分子系统进化关系。方法对无锡市淡色库蚊、三带喙库蚊、二带喙库蚊、小拟态库蚊和褐尾库蚊的COⅠ基因进行扩增、测序,在GenBank中对所测序列进行同源性比对,分析基因特征、颠换率、分化年代,利用Mega 4.1软件构建分子系统树。结果小拟态库蚊的COⅠ基因为首次测序;5种库蚊的COⅠ基因序列长度为692～717 bp,GC含量为29.91%～31.42%,颠换率为5.610%～8.555%,分化年代为2.44×10⁶a～3.72×10⁶a;每种蚊虫为单系群的支持值均≥98。结论 COⅠ基因具有种间特异性,可用于5种库蚊的分类鉴别。

关键词: 库蚊, 细胞色素氧化酶亚基Ⅰ基因, 分子进化

CLC Number:

R384.1

LAN Ce-jie, SHEN Yuan, LU Liang, MA Guang-yuan, WU Jia-lin. Molecular evolution analysis of COⅠ gene in five species of Culex[J]. Chines Journal of Vector Biology and Control, 2013, 24(1): 28-30.

兰策介, 沈元, 鲁亮, 马广源, 吴家林. 5种库蚊的COⅠ基因分子进化分析[J]. 中国媒介生物学及控制杂志, 2013, 24(1): 28-30.

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Molecular evolution analysis of COⅠ gene in five species of Culex

5种库蚊的COⅠ基因分子进化分析

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