Molecular epidemiological investigation on hantavirus carried by rodents in Fujian port

Abstract

Abstract: Objective To identify the genotype and analyze molecule characteristics of main Hantavirus in Fujian port. Methods Nested RT-PCR was used to detect Hantavirus-specific nucleic acid sequences in rodents captured in Fujian port, and M segment was sequenced and analyzed in positive samples. Results A total of 839 rodents were trapped in Fujian port in 2010. Rattus norvegicus was the dominant species accounting for 58.52%. The positive rate of Hantavirus in captured rodents was 5.84%, which were all of Seoul Virus (SEOV). Hantavirus positive species were mainly R. norvegicus (8.96%) and R. tanezumi (3.09%). Generally, Hantavirus infection significantly increased during winter and spring. The phylogenetic analysis based on partial M segment (nt2001-2301) sequence in 25 samples indicated that there were two genetic subtypes, Seoul 2 and Seoul 3. Conclusion Seoul 3 is the main hantavirus subtype carried by rodents in Fujian port.

Key words: Rodent, Hantavirus, Genotype, Phylogenetic tree

摘要： 目的了解福建口岸汉坦病毒的基因型别和分子特征。方法对2010年福建口岸捕获的鼠形动物进行汉坦病毒检测,对部分阳性标本进行M基因片段序列分析。结果 2010年福建口岸共捕获鼠形动物839只,以褐家鼠为优势鼠种,占58.52%;鼠形动物携带汉坦病毒阳性率5.84%,均为汉城型(SEO),阳性鼠种为褐家鼠和黄胸鼠,阳性率分别为8.96%和3.09%。汉坦病毒检出呈较明显的冬春季高峰。用M基因片段(nt2001～2301)核苷酸序列构建系统进化树,将福建口岸SEOV分为S2和S3两个亚型。结论 S3亚型SEOV为福建口岸优势流行亚型。

关键词: 啮齿类动物, 汉坦病毒, 基因型, 系统进化树

CLC Number:

WANG Yu-ping, XU Qing, CAI Heng-zhong, CHEN Jun, ZHANG Jian-ming, ZHENG Yan-ping, GAO Bo. Molecular epidemiological investigation on hantavirus carried by rodents in Fujian port[J]. Chines Journal of Vector Biology and Control, 2012, 23(4): 292-294.

王宇平, 许卿, 蔡亨忠, 陈钧, 张建明, 郑燕平, 高博. 福建口岸啮齿类动物携带汉坦病毒分子流行病学调查[J]. 中国媒介生物学及控制杂志, 2012, 23(4): 292-294.

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