Abstract:

Objective To get an insight into the resistance mechanisms of Musca domestica to beta-cypermethrin so that a theoretical basis was provided for the selection of a rapid and accurate monitoring technique and for the scientific use of insecticides. Methods The glutathione peroxidase(GSH-PX) activities of three strains of M. domestica cultured in laboratory, the beta-cypermethrin resistant strains, the resistance decay strains and the sensitive strains was detected by SDS-PAGE analysis to evaluate the role of the peroxisome in the development of M. domestica resistance. Results It was found that GSH-PX activity of the resistant M. domestica increased significantly with a relative activity of 165.89% in contrast with the activity of the enzyme that decreased to 70.13% in the resistance decay strains. Significant differences were visualized in 6 bands of the electrophoresis pattern of the 3 strains. Conclusion The change in the peroxidase activity in M. domestica strains resistant to beta-cypermethrin may play an important role in the development of M. domestica resistance.

Key words: Musca domestica, Glutathione?peroxidase, Activity, SDS?PAGE

摘要：

目的 研究家蝇抗药性产生机制，为选择快速准确的监测技术和科学用药提供理论依据。方法 通过对实验室培育的抗高效氯氰菊酯品系、抗性衰减品系及敏感品系家蝇的谷胱甘肽-过氧化物酶(GSH-PX)的活性及SDS-聚丙烯酰胺凝胶电泳分析，观察过氧化物酶对家蝇抗性产生的作用。结果 抗性家蝇GSH-PX的活性明显增高，抗性品系家蝇相对活性达到165.89%，抗性衰减品系活性降低到70.13%。3个家蝇品系的电泳图谱中6组条带有较明显的差别。结论 抗高效氯氰菊酯家蝇的过氧化物酶性质发生改变，对家蝇的抗药性产生可能起比较重要的作用。

关键词: 家蝇, 谷胱甘肽-过氧化物酶, 酶活性, SDS-聚丙烯酰胺凝胶电泳