Abstract:

Objective To identify the status quo of Lyme infections among the population, vector ticks and host animal rats in the forest region of Changbai and Tonghua counties, Jinlin province. Methods Serological samples were collected in the forest area in Changbai and Tonghua. Ticks and rats were captured at random sites. Indirect immunofluorescence assay (IFA) was used for serological detection and nested-PCR for etiological detection of tick and rodent samples as well as gene sequencing typing. Pathogenic isolation and culture was done using BSK culture media. Results The seroprevalence of Borrelia burgdorferi IgG was 12.04% (36/299) in human in Changbai and 9.35% (23/246) in Tonghua. The carrying rate in ticks was 27.08% and 20.41% in Changbai (39/144) and Tonghua (20/98), respectively, while the carrying rate in rodents was 10.00% in both counties (4/40, 1/10). The sequence homology analysis showed that the ticks and rats were carrying the genotype Borrelia garinii. Conclusion Lyme infection was serologically evident in human in Changbai and Tonghua’s forest areas. Etiological Results showed that Ixodes persulcatus and Apodemus agrarius were the key vector and host of Lyme disease in this region.

Key words: Borrelia burgdorferi, Serology, Tick, Rat, Etiological detection

摘要：

目的 了解吉林省长白及通化县林区人群、生物媒介蜱和宿主动物鼠的莱姆病螺旋体感染情况。方法 在长白及通化县林区采集人群血清，并随机选择采样点进行捕蜱、捕鼠。采用间接免疫荧光法(IFA)检测血清，巢式PCR对蜱、鼠标本进行病原学检测，并通过基因测序方法确定基因型。同时蜱、鼠标本接种BSK培养基进行病原分离培养。结果从长白及通化县检查到人群血清莱姆病抗体IgG阳性率，长白县为12.04%(36/299)，通化县为9.35%(23/246)；蜱的带菌率，长白县为27.08%(39/144)，通化县为20.41%(20/98)；鼠的带菌率，长白及通化县均为10.00%(4/40，1/10)。通过序列同源性分析确定蜱和鼠携带的莱姆病螺旋体基因型存在Borrelia garinii。结论 从血清学上证实长白及通化林区人群存在莱姆病螺旋体的感染，从病原学上证实全沟硬蜱和黑线姬鼠是当地莱姆病螺旋体的重要传播媒介和宿主。

关键词: 莱姆病螺旋体, 血清学, 蜱, 鼠, 病原学检测