Abstract:

Objective To monitor the dynamics of 10 pathogens carried by the local cockroaches and the cockroaches imported through Shanghai Port. Methods The target pathogens were isolated and cultured, and subject to biochemical identification and the PCR test. Results Pathogens detected from the sample cockroaches included mold, Escherichia coli, Pseudomonas aeruginosa and hepatitis B virus, while Salmonella, Vibrio parahaemolyticus, Vibrio cholerae, Staphylococcus aureus, Mycobacterium tuberculosis and Shigella were not detected. There was no significant difference in the carrying rate among the 4 species of cockroach (χ²=6.20, P>0.05). Conclusion Cockroaches are the intermediate host of pathogens. Therefore, advanced techniques should be used at ports to detect pathogens carried by them.

Key words: Cockroaches, Pathogen, Detection

摘要：

目的 监测上海口岸输入性及本地蜚蠊携带的10种病原体情况。方法 采用分离培养、生化鉴定、PCR检测病原体。结果 从蜚蠊中检出的病原体有霉菌、大肠埃希菌、绿脓杆菌和乙型肝炎病毒；未检出沙门菌、副溶血性弧菌、霍乱弧菌、金黄色葡萄球菌、结核杆菌和志贺菌。4类蜚蠊病原体携带率差异无统计学意义(χ²=6.20，P>0.05)。结论 蜚蠊是病原体扩散的中间宿主，口岸应采用先进检测技术监测蜚蠊中病原体。

关键词: 蜚蠊, 病原体, 检测