Abstract:

Objective　To evaluate the insecticidal resistance of Culex pipiens pallens and Musca domestica in Zhoushan city for providing the basis for studying the mechanism of resistance development in mosquitoes and flies and developing control and prevention measures. Methods　The median lethal concentration (LC₅₀) for Cx. pipiens pallens larvae was determined by the impregnation method; the median lethal dose (LD₅₀) for M. domestica was determined by the topical application method. Results　The  resistance  coefficients  of  Cx. pipiens pallens  natural   strains  were  between  1.5  and  10.8  to  the  following  insecticides in descending order: 10.8 to beta?cypermethrin, 9.4 to permethrin, 5.3 to deltamethrin, 4.3 to tetramethrin, 4.1 to dichlorvos, and 1.5 to BPMC. The resistance coefficients of M. domestica natural strains were between 4.4 and 29.9 to the following insecticides in descending order: 29.9 to deltamethrin, 23.6 to beta?cypermethrin, 17.2 to cypermethrin, 8.8 to permethrin, 8.4 to dichlorvos, and 4.4 to tetramethrin. Conclusion　The Cx. pipiens pallens and M. domestica in Zhoushan city demonstrated different degrees of resistance to the six insecticides; the resistance to pyrethroid insecticides was higher than to the other types, which may be related to the strategy of insecticidal application in the city.

Key words: Culex pipiens pallens, Musca domestica, Insecticidal resistance, Impregnation method, Topical application

摘要：

目的　了解舟山市淡色库蚊和家蝇的抗药性水平，为蚊、蝇抗性产生机制的研究及防制措施制定提供科学依据。方法　采用浸渍法测定淡色库蚊幼虫半数致死浓度（LC₅₀）；采用点滴法测定家蝇半数致死剂量（LD₅₀）。结果　淡色库蚊自然种群抗性系数在1.5～10.8倍之间，依次为高效氯氰菊酯10.8倍，氯菊酯9.4倍，溴氰菊酯5.3倍，胺菊酯4.3倍，敌敌畏4.1倍，仲丁威1.5倍。家蝇自然种群抗性系数在4.4～29.9倍之间，依次为溴氰菊酯29.9倍，高效氯氰菊酯23.6倍，氯氰菊酯17.2倍，氯菊酯8.8倍，敌敌畏8.4倍，胺菊酯4.4倍。结论　舟山市淡色库蚊和家蝇对6种杀虫剂均产生了不同程度的抗性，拟除虫菊酯类杀虫剂的抗性水平高于其他类杀虫剂，可能与该市的化学杀虫剂用药策略有关。

关键词: 淡色库蚊, 家蝇, 抗药性, 浸渍法, 点滴法