Abstract:

Objective　Borrelia burgdorferi were isolated from captured ticks in the border areas between Guangxi and Guizhou, in order to identify the vector of Lyme disease in Guangxi Zhuang Autonomous Region and Guizhou province. Methods　The BSK?Ⅱ culture media were used to isolate B. burgdorferi from ticks. The 5S-23S rRNA gene spacer region of the isolates was amplified by the PCR, and the product was cloned and sequenced. The sequencing results were compared with the 5S-23S rRNA gene spacer region of the B. burgdorferi strains from the gene bank and analyzed for the homology to identify their genotypes. Results　One B. burgdorferi was isolated from the captured Ixodes sinensis (named QLT1). The isolate was identified as the genotype B. valaisiana. Conclusion　I. sinensis is the likely vector of Lyme disease in Guangxi Zhuang Autonomous Region and Guizhou province.

Key words: Lyme disease, Borrelia burgdorferi, Ixodes sinensis, Genotype

摘要：

目的 　从桂黔交界地区捕获的蜱中分离莱姆病螺旋体，以探查广西壮族自治区（广西区）和贵州省莱姆病的传播媒介。方法　用BSK-Ⅱ培养基从蜱中分离莱姆病螺旋体。用PCR扩增分离菌株的5S～23S rRNA基因间隔区，将PCR扩增产物克隆后测序，通过将测序结果与基因库中莱姆病螺旋体菌株的5S～23S rRNA基因间隔区进行同源性比较和分析，从而鉴定分离株的基因型。结果　从捕获的中华硬蜱中分离出1株莱姆病螺旋体（命名为QLT1）。分离株经鉴定为Borrelia valaisiana基因型莱姆病螺旋体。结论　中华硬蜱很有可能是广西区和贵州省莱姆病的传播媒介。

关键词: 莱姆病, 伯氏疏螺旋体, 中华硬蜱, 基因型