Molecular epidemiological investigation of Bartonella infection in ticks in Zhejiang province

Chines Journal of Vector Biology and Control ›› 2010, Vol. 21 ›› Issue (3): 232-234.

• Original reports • Previous Articles Next Articles

Molecular epidemiological investigation of Bartonella infection in ticks in Zhejiang province

SUN Ji-Min¹, LU Liang², LIU Qi-Yong², LING Feng¹, WANG Zhen¹, LIN Jun-Fen¹, CHAI Cheng-Liang¹, LV Hua-Kun¹, FU Gui-Ming¹

1 Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China; 2 National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention

Received:2009-11-20 Online:2010-06-20 Published:2010-06-20
Supported by:
Supported by the Zhejiang Center for Disease Control and Prevention Youth and Technology Fund

浙江省蜱中巴尔通体感染的分子流行病学调查

孙继民¹，鲁亮²，刘起勇²，凌锋¹，王臻¹，林君芬¹，柴程良¹，吕华坤¹，傅桂明¹

1 浙江省疾病预防控制中心传染病防制所（杭州 310051）； 2 中国疾病预防控制中心传染病预防控制所

作者简介:孙继民（1981-），男，医师，主要从事自然疫源性疾病的监测及疫情控制。
基金资助:
浙江省疾病预防控制中心青年科技基金

Abstract

Abstract:

Objective　The dominant species of ticks in Zhejiang province was identified and detected for Bartonella infection in order to provide the basis for the control and prevention of Bartonella human infection. Methods　Engorged adult ticks were collected from the body surface of the animals in Tiantai, Jindong and Jiangshan areas, and the prevalence of Bartonella was detected using PCR. Sequencing analysis was then performed to identify the Bartonella species. Results　All captured ticks were Ixodes sinensis, with the positive rates of 42.3%(11/26), 6.7%(2/30) and 3.3%(1/30) in Tiantai, Jindong and Jiangshan areas, respectively. The average positive rate was 16.3%. The detected Bartonella had the closest genetic relationship with Bartonella rattimassilensis, followed by the human pathogenic B. grahamii. Conclusion　The presence of Bartonella infection in ticks in Zhejiang posed the risk of transmission to humans, and hence the corresponding control measures should be taken.

Key words: Tick, Bartonella, Molecular epidemiology

摘要：

目的　了解浙江省的优势蜱种，检测其巴尔通体感染情况，为巴尔通体的预防控制提供科学依据。方法　从天台、金东和江山地区的动物体表采集饱血成蜱，用PCR方法检测其阳性率，并克隆测序后分析所检测到的巴尔通体种类。结果　所捕获的蜱均为中华硬蜱，天台、金东和江山地区的阳性率分别为42.3%（11/26）、6.7%（2/30）和3.3%（1/30），平均阳性率为16.3%，所检测到的巴尔通体与Bartonella rattimassilensis的遗传关系最近，与人类致病性巴尔通体B. grahamii的遗传关系也很近。结论　浙江省蜱中存在巴尔通体感染，具有传播给人的风险，应该采取措施加以控制。

关键词: 蜱, 巴尔通体, 分子流行病学

CLC Number:

SUN Ji-Min, LU Liang, LIU Qi-Yong, LING Feng, WANG Zhen, LIN Jun-Fen, CHAI Cheng-Liang, LV Hua-Kun, FU Gui-Ming. Molecular epidemiological investigation of Bartonella infection in ticks in Zhejiang province[J]. Chines Journal of Vector Biology and Control, 2010, 21(3): 232-234.

孙继民1，鲁亮2，刘起勇2，凌锋1，王臻1，林君芬1，柴程良1，吕华坤1，傅桂明1. 浙江省蜱中巴尔通体感染的分子流行病学调查[J]. 中国媒介生物学及控制杂志, 2010, 21(3): 232-234.

References

［1］ Boulouis HJ, Chang CC, Henn JB, et al. Factors associated with rapid emergence of zoonotic Bartonella infections［J］. Vet Res，2005，36（2005）：383-410.
［2］ Jennifer BH, Mourad HW, Rickie WK, et al. Gray Foxes （Urocyon cinereoargenteus） as a potential reservoir of a Bartonella clarridgeiae?like bacterium and domestic dogs as part of a sentinel system for surveillance of zoonotic arthropod?borne pathogens in northern California［J］. J Clin Microbiol，2007，45（8）：2411-2418.
［3］ Norman AF, Regnery R, Jameson P, et al. Differentiation of Bartonella?like isolates at the species level by PCR?restriction fragment length polymorphism in the citrate synthase gene［J］. J Clin Microbiol，1995，33（7）：1797-1803.
［4］栗冬梅，俞东征，刘起勇，等. 用分子生物学技术诊断巴尔通体感染［J］. 中华流行病学杂志，2004，25（7）：602-606.
［5］ Birtles RJ, Raoult D. Comparison of partial citrate synthase gene （gltA） sequences for phylogenetic analysis of Bartonella［J］. Int J Syst Bacteriol，1996，46（4）：891-897.
［6］ Adelson ME, Rao RV, Tilton RC, et al. Prevalence of Borrelia burgdorferi, Bartonella spp., Babesiamicroti, and Anaplasma phagocytophila in Ixodes scapularis ticks collected in northern New Jersey［J］. J Clin Microbiol，2004，42（6）：2799-2801.
［7］ Chang CC, Chomel BB, Kasten RW, et al. Molecular evidence of Bartonella spp. in questing adult Ixodes pacificus ticks in California ［J］. J Clin Microbiol，2001，39（4）：1221-1226.
［8］ Sanogo YO, Zeaiter Z, Caruso G, et al. Bartonella henselae in Ixodes ricinus ticks（Acari：Ixodida） removed from humans, Belluno province, Italy［J］. Emerg Infect Dis，2003，9（3）：329-332.
［9］ Schouls LM, Van De Poll, Rijpkema SG, et al. Detection and identification of Ehrlichia， Borrelia burgdorferi sensu lato, and Bartonella species in dutch Ixodes ricinus ticks［J］. J Clin Microbiol，1999，37（7）：2215-2222.
［10］ Parola P, Shpynov S, Montoya M, et al. First molecular evidence of new Bartonella spp. in fleas and a tick from Peru［J］. Am J Trop Med Hyg，2002，67（2）：135-136.
［11］栗冬梅，刘起勇，俞东征，等. 蚤蜱中巴尔通体的分离培养及检测鉴定［J］. 中国人兽共患病学报，2005，21（12）：1052-1058.

[1]	WEI Zi-xin, FANG Yuan, ZHANG Yi. Ticks species， distribution and pathogens in Shanghai， China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 120-124.
[2]	XIANG Yu-long, ZHOU Jing-zhu, LIU Ying, LIU Ping-tao, HU Yong, LIANG Wen-qin. An investigation of ticks and tick-borne bacteria in some areas of Guizhou province， China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 148-152.
[3]	WANG Yan-hua, SUN Ying-wei, MAO Ling-ling, WANG Zi-jiang, ZHANG Jia-yong, ZHANG Ji-bo, PENG Yao, XIA Lian-xu. Detection of Francisella strains and Francisella-like endosymbionts in ticks in some regions of China [J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 756-762.
[4]	XU Xue-lian, HAN A-xiang, YE Shi-qing, GUAN Wan-chun, LOU Yong-liang. Metagenomic analysis of microbial community structure, antibiotic resistance genes, and virulence factors of ticks captured at Wenzhou port, Zhejiang province, China [J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 763-771.
[5]	YANG Xiao-na, ZHANG Lin, HOU Xue-xia, HAO Qin. Application of 16S rDNA full-length high-throughput sequencing in the study of tick-borne pathogen biodiversity [J]. Chines Journal of Vector Biology and Control, 2021, 32(4): 404-411.
[6]	WEI Na-na, ZHANG Hou-shuang, ZHOU Jin-lin. Research progress in epidemic situation and control techniques of tick-borne diseases in pets [J]. Chines Journal of Vector Biology and Control, 2021, 32(3): 257-264.
[7]	SHI Jia-jia, LYU Ming-jie, YUAN Zhong-liang. An investigation of geographical distribution, host, and seasonality of ticks in Zhengzhou, China [J]. Chines Journal of Vector Biology and Control, 2021, 32(3): 361-364.
[8]	LI Ji-xu, PIAO Wen, JIN Guang-jun. Transovarial transmission of a new genotype Candidatus Rickettsia longicornii of spotted fever group Rickettsia in Haemaphysalis longicornis [J]. Chines Journal of Vector Biology and Control, 2021, 32(2): 139-143.
[9]	ZHANG Meng, WANG Tong-xuan, YU Zhi-jun, LIU Jing-ze. An overview of tick removal methods [J]. Chines Journal of Vector Biology and Control, 2021, 32(2): 243-246.
[10]	MA Jing, GAO Jin-liang. Research advances in anticoagulant mechanisms of tick-derived protease inhibitors containing the Kunitz domain [J]. Chines Journal of Vector Biology and Control, 2021, 32(1): 111-114.
[11]	SHEN Bo, LI Ji-xu, HUANG Biao, ZHAO Qing-long, YAO Lai-shun, WANG Ao, WU Dong-lin, YANG Xian-da, WEI Lei-lei, DENG Li-quan. An investigation of tick species in Changbai mountain area of Jilin province, China [J]. Chines Journal of Vector Biology and Control, 2020, 31(6): 667-671.
[12]	ZHANG Yu-fu, ZHANG Shou-gang, ZHU Ding, GE Xiao-wu, CAO Gan, CHU Hong-liang. Comparative analysis of cockroach infestation rate based on sticky trap method and visual observation [J]. Chines Journal of Vector Biology and Control, 2020, 31(6): 676-679.
[13]	YUE Yu-juan, REN Dong-sheng, WU Hai-xia, LIU Xiao-bo, ZHAO Ning, GUO Yu-hong, LI Gui-chang, WANG Xue-shuang, SONG Xiu-ping, WANG Jun, LU Liang, LIU Qi-yong. National surveillance report on cockroaches in China, 2019 [J]. Chines Journal of Vector Biology and Control, 2020, 31(4): 412-416.
[14]	WU Hai-xia, LIU Xiao-bo, YUE Yu-juan, REN Dong-sheng, WANG Jun, ZHAO Chun-chun, LI Gui-chang, ZHAO Ning, SONG Xiu-ping, LIU Qi-yong. National surveillance report on ticks in China, 2019 [J]. Chines Journal of Vector Biology and Control, 2020, 31(4): 417-422.
[15]	ZHANG Lin, WEN Shu, HOU Xue-xia, LI Sha, YANG Xiao-na, CHEN Ting, MIAO Guang-qing, FU Xiao-ying, HAO Qin, ZHU Xiong. Detection and genotyping of Borrelia burgdorferi in parasitic ticks in Qiongzhong area of Hainan province, China [J]. Chines Journal of Vector Biology and Control, 2020, 31(4): 426-429.

Molecular epidemiological investigation of Bartonella infection in ticks in Zhejiang province

浙江省蜱中巴尔通体感染的分子流行病学调查

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments