Abstract: Amopheles minimus is one of the main vectors of malaria in southeast Asia. Recent studies have been continued on morphology, ecology, genetic,esterase isozyme and contagion of plasmodiums.In this paper,random amplified polymorphicDNA(RAPDs)were used to estimate genetic relationships and identification of 30 Anopheles minimus from geographic population in Thaland, Guangxi province and lingcang China. Molecular phylogenetic tree derived from genetic distance suggested that the RAPDs are most related to geographic condition. There were genetic divergences among different geographic population. There results indicate that the RAPD technique will be useful in studies of mosquito molecular toxonomy and in epidemiologic studies of the relatedness of geographic population and vector movement.

摘要： 目的：对来自泰国、广西、临沧3个地区的30只雌蚊进行随机扩增多态DNA分析。方法：RAPD法。结果：UPGMA法构建的分子系统树表明，30只微小按蚊可归并为显著不同的3个组，证明各群体间存在着明显不同的基因组多态性基因流，不同群体存在着显著的遗传分化。结论：用RAPD可以区分不同地理群体的微小按蚊。

关键词: RAPD, 微小按蚊, 种群