ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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Jiang Lian-hua ; Zhao Chun-sheng ; Liu Jin-hua et al
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蒋廉华; 赵春生; 刘金华; 方美玉; 徐春华; 陈唯军; 陆振豸; 饶颐年
Abstract: In 1995,14 strains arbovirus were isolated from four species of mosquitoes collected in Hainan province.These isolates could grow in C6/36 cells and Vero-E6 cells,and showed marked CPE.They could induce illness,and were sensitive to acid and ether but resistant to 5-FudR.These viruses were identified primarily to belong to 8 strains flaviviruses and 6 strains alphavirus by serology ways and the reverse transcription and polymerase chain reaction (RTPCR).
摘要: 目的:鉴定海南省虫媒病毒分离物。方法:现场捕蚊,分离与鉴定虫媒病毒,主要方法为逆转录聚合酶链反应等。结果:1995年从海南省琼中县大丰农场、三亚市立才农场、万宁县兴隆农场、乐东县尖峰林场及西沙捕获的4种蚊虫中分离到14株虫媒病毒,均可在C6/36细胞、Vero-E6细胞组织培养中增殖,并产生明显的病变;对酸、乙醚敏感,抵抗5-碘脱氧尿苷,属RNA病毒。结论:经血清学和逆转录-聚合酶链反应(RT-PCR)初步鉴定,有8株为黄病毒科病毒,6株为披膜病毒科甲病毒。
关键词: 黄病毒, 甲病毒, 逆转录-聚合酶链反应
Jiang Lian-hua ; Zhao Chun-sheng ; Liu Jin-hua et al. Preliminary Identification on Arbovirus Isolates from Hainan Province[J]. Chines Journal of Vector Biology and Control.
蒋廉华; 赵春生; 刘金华; 方美玉; 徐春华; 陈唯军; 陆振豸; 饶颐年. 海南省虫媒病毒分离物的初步鉴定[J]. 中国媒介生物学及控制杂志.
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