关键词: 白纹伊蚊, 幼虫浸渍法, 成蚊接触筒法, 抗药性

Abstract: Objective To investigate the resistance level of Aedes albopictus to commonly used insecticides in Weifang,Shandong, China, so as to provide a reference for the scientific use of insecticides.Methods The larval dipping method was used to determine larval deaths, the SPSS 19 software was used to caculate the toxic regression equation and the median lethal concentration (LC₅₀) of insecticides on the larvae at the late 3rd instar and the early 4th instar, and WHO tube method was used to measure the 24 h mortality rate of healthy female mosquitoes without blood-sucking.Results The larvae of Ae. albopictus showed a resistance ratio of 3.07-6.52 fold to permethrin,propoxur,and temephos and a resistance ratio of 12.40-18.40 fold to Bacillus thuringensis israelensis (Bti, 7 000 ITU/mg). Adult mosquitoes developed resistance to 0.08% beta-cypermethrin, 0.4% permethrin,0.07% lambda-cyhalothrin,and 0.03% deltamethrin, with a 24 h mortality rate of 15.94%-62.32%. The mosquitoes were determined as possibly resistant to 0.2% fenitrothion in 2022 (with a mortality rate of 92.65%) and were determined as sensitive to 0.5% malathion, 2% chlorpyrifos, 0.2% fenitrothion, 0.2% bendiocarb, and 0.05% propoxur, with a mortality rate of 98.63%-100%.Conclusions Ae. albopictus mosquitoes in Weifang have developed serious resistance to most pyrethroid insecticides. Therefore,local use of insecticides for the prevention and control of mosquitoes should be adjusted according to surveillance results of insecticide resistance, and integrated measures, mainly environmental management, should be implemented. In addition, different insecticides should be used alternatively or in combination, so as to prevent and delay the development of insecticide resistance.

Key words: Aedes albopictus, Larvae dipping method, WHO tube method, Insecticide resistance