内蒙古自治区森林革蜱感染立克次体、无形体及埃立克体的研究

doi:10.11853/j.issn.1003.8280.2023.04.021

中国媒介生物学及控制杂志 ›› 2023, Vol. 34 ›› Issue (4): 559-563.DOI: 10.11853/j.issn.1003.8280.2023.04.021

内蒙古自治区森林革蜱感染立克次体、无形体及埃立克体的研究

张晨曦¹(), 武少卿²(), 刘洋³, 刘芳⁴, 王宇⁵, 范蒙光⁴^,*(), 李建云⁴^,*()

1. 内蒙古医科大学公共卫生学院, 内蒙古呼和浩特 010110
2. 乌兰察布市疾病预防控制中心, 内蒙古乌兰察布 012000
3. 包头医学院, 内蒙古包头 014000
4. 内蒙古自治区综合疾病预防控制中心, 内蒙古呼和浩特 010031
5. 内蒙古农业大学, 内蒙古呼和浩特 010018

收稿日期:2023-03-02 出版日期:2023-08-20 发布日期:2023-08-17
通讯作者: 范蒙光,李建云
作者简介:张晨曦，在读硕士，主要从事流行病学与分子流行病学研究工作，E-mail：zcx125430@163.com
武少卿，男，微生物检验师（初级），主要从事鼠疫监测与实验室检验，E-mail：454902088@qq.com
第一联系人：
张晨曦、武少卿为共同第一作者
基金资助:
内蒙古自治区科技重大专项(2021ZD0006);内蒙古自治区自然科学基金(2018MS08059);国家重点研发计划(2021YFC1200200)

An analysis of Rickettsia, Anaplasma, and Ehrlichia infections in Dermacentor silvarum in Inner Mongolia Autonomous Region, China

Chen-xi ZHANG¹(), Shao-qing WU²(), Yang LIU³, Fang LIU⁴, Yu WANG⁵, Meng-guang FAN⁴^,*(), Jian-yun LI⁴^,*()

1. School of Public Health, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010110, China
2. Ulanqab Center for Disease Prevention and Control, Ulanqab, Inner Mongolia 012000, China
3. Baotou Medical College, Baotou, Inner Mongolia 014000, China
4. Inner Mongolia Center for Comprehensive Disease Prevention and Control, Hohhot, Inner Mongolia 010031, China
5. Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China

Received:2023-03-02 Online:2023-08-20 Published:2023-08-17
Contact: Meng-guang FAN, Jian-yun LI
Supported by:
Inner Mongolia Science and Technology Major Project of China(2021ZD0006);Natural Science Foundation of Inner Mongolia Autonomous Region of China(2018MS08059);National Key R&D Program of China(2021YFC1200200)

摘要/Abstract

摘要：

目的: 了解我国内蒙古自治区（内蒙古）森林革蜱感染立克次体、无形体及埃立克体等病原体情况，并研究其遗传多样性。方法: 在内蒙古乌兰察布市四子王旗、呼和浩特市土默特左旗家畜体表采集寄生蜱成蜱76只，经形态学和分子生物学鉴定后，用针对16S rRNA基因和柠檬酸合成酶基因（gltA）保守区域的引物同时检测立克次体，用针对16S rRNA基因的属特异性引物检测埃立克体、无形体。对检出的阳性样本，使用巢式PCR扩增获得gltA和热休克蛋白基因（groEL）。用PhyML 3.0软件进行最大似然法进化树构建。结果: 采获蜱经鉴定均为森林革蜱。从上述采集蜱中检出立克次体阳性8份，其中四子王旗6份（6/35，17.14%），土默特左旗2份（2/41，4.88%），测序结果显示均为劳氏立克次体。对3个基因序列的进化分析，上述菌株分成2个不同的分支，当地流行的劳氏立克次体有一定遗传多样性。未检出埃立克体和无形体。结论: 内蒙古的革蜱中存在劳氏立克次体的流行，且存在一定遗传多样性。劳氏立克次体为人类病原体，在内蒙古局部地区较高的阳性率提示可能存在感染人的风险。

关键词: 内蒙古, 四子王旗, 土默特左旗, 森林革蜱, 劳氏立克次体

Abstract:

Objective: To investigate the situation of Rickettsia, Anaplasma, and Ehrlichia infections in Dermacentor silvarum and their genetic diversity in the Inner Mongolia Autonomous Region (Inner Mongolia), China. Methods: A total of 76 adult parasitic ticks were collected from the body surface of livestock in Dorbod Banner in Ulanqab and Tumed Left Banner in Hohhot, Inner Mongolia. All of them were morphologically and molecularly identified. Rickettsia in the ticks was examined using primers targeting the conserved regions of both 16S rRNA and citrate synthase (gltA) genes. Anaplasma and Ehrlichia were examined using genus-specific primers targeting the 16S rRNA gene. For positive specimens, nested PCR was used to amplify the gltA gene and the heat shock protein gene (groEL). PhyML 3.0 software was used for constructing maximum likelihood phylogenetic trees. Results: All the captured ticks were identified as D. silvarum. A total of 8 ticks tested positive for Rickettsia, with six (6/35, 17.14%) from Dorbod Banner and two (2/41, 4.88%) from Tumed Left Banner, all being identified as R. raoultii by sequencing. The phylogenetic analysis based on the 16S rRNA, gltA, and groEL genes revealed that the strains were divided into two clades in the phylogenetic tree, suggesting that R. raoultii was genetically diverse in this area. All the samples were negative for Anaplasma and Ehrlichia. Conclusions: R. raoultii is epidemic among Dermacentor ticks in Inner Mongolia, with certain genetic diversity. Concerning its human pathogenicity, the high positive rate of R. raoultii in certain areas of Inner Mongolia suggests a potential risk of human infections.

Key words: Inner Mongolia, Dorbod Banner, Tumed Left Banner, Dermacentor silvarum, Rickettsia raoultii

中图分类号:

R384.4
R376

张晨曦, 武少卿, 刘洋, 刘芳, 王宇, 范蒙光, 李建云. 内蒙古自治区森林革蜱感染立克次体、无形体及埃立克体的研究[J]. 中国媒介生物学及控制杂志, 2023, 34(4): 559-563.

Chen-xi ZHANG, Shao-qing WU, Yang LIU, Fang LIU, Yu WANG, Meng-guang FAN, Jian-yun LI. An analysis of Rickettsia, Anaplasma, and Ehrlichia infections in Dermacentor silvarum in Inner Mongolia Autonomous Region, China[J]. Chinese Journal of Vector Biology and Control, 2023, 34(4): 559-563.

图/表 2

表 1 立克次体、无形体、埃立克体PCR反应条件

Table 1 PCR reaction conditions for Rickettsia, Anaplasma, and Ehrlichia

图 1 内蒙古森林革蜱携带立克次体遗传分析与系统进化分析注：红色和绿色部分为本研究获得的阳性菌株；gltA柠檬酸合成酶基因；groEL热休克蛋白基因。

Fig. 1 Genetic analysis and phylogenetic analysis of Rickettsia borned by Dermacentor silvarum in Inner Mongolia, China

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内蒙古自治区森林革蜱感染立克次体、无形体及埃立克体的研究

An analysis of Rickettsia, Anaplasma, and Ehrlichia infections in Dermacentor silvarum in Inner Mongolia Autonomous Region, China

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