盐酸环丙沙星对家蝇幼虫和蛹能量物质含量的影响

doi:10.11853/j.issn.1003.8280.2019.05.008

中国媒介生物学及控制杂志 ›› 2019, Vol. 30 ›› Issue (5): 514-518.DOI: 10.11853/j.issn.1003.8280.2019.05.008

盐酸环丙沙星对家蝇幼虫和蛹能量物质含量的影响

万晴^1,2, 宋暖^1,3, 黄振东^1,2, 薛志静^1,2, 庄桂芬^1,2, 张瑞玲^1,2, 许永玉³, 张忠^1,2

1 泰山医学院基础医学院, 山东泰安 271016;
2 山东省新发传染病溯源与防控协同创新中心, 山东泰安 271016;
3 山东农业大学植物保护学院, 山东泰安 271000

收稿日期:2019-04-05 出版日期:2019-10-20 发布日期:2019-10-20
通讯作者: 张忠,Email:nasonia@163.com
作者简介:万晴,女,在读硕士,主要从事病媒生物控制研究,Email:2784479622@qq.com
基金资助:
国家自然科学基金（81572028，81401693，81271874）

Effect of ciprofloxacin hydrochloride on energy substances in the larvae and pupae of Musca domestica

WAN Qing^1,2, SONG Nuan^1,3, HUANG Zhen-dong^1,2, XUE Zhi-jing^1,2, ZHUANG Gui-fen^1,2, ZHANG Rui-ling^1,2, XU Yong-yu³, ZHANG Zhong^1,2

1 College of Basic Medical Science of Taishan Medical University, Tai'an 271016, Shandong Province, China;
2 Shandong Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases;
3 Plant Protection College of Shandong Agricultural University

Received:2019-04-05 Online:2019-10-20 Published:2019-10-20
Supported by:
Supported by the Natural Science Foundation of China (No. 81572028, 81401693, 81271874)

摘要/Abstract

摘要： 目的研究盐酸环丙沙星对家蝇幼虫和蛹体内能量物质含量的影响。方法 2013年6-9月，采用实验室家蝇种群作为试虫，幼虫孵化后，提供常规饲料作为对照组，含0.1%、0.3%和0.5%盐酸环丙沙星的饲料作为实验组。测定能量物质采样时，1龄幼虫采用直接研磨法取样，2~3龄幼虫和蛹提取血淋巴作为样品。分别采用考马斯亮蓝法测定蛋白质含量，蒽酮-浓硫酸比色法测定总糖含量，磷酸香草醛显色反应法测定脂肪含量。SPSS 20.0软件用于统计学分析，组间比较采用单因素方差分析，独立样本两两比较采用t检验。结果 0.5%盐酸环丙沙星组幼虫和蛹体内蛋白质含量最高可下降为对照组的4.00%和10.00%；0.1%、0.3%和0.5%组幼虫体内脂肪含量下降约为对照组的75.00%、65.00%和50.00%，蛹体内脂肪含量下降约为对照组的85.00%、75.00%和50.00%；总糖含量均下降约为对照组的80.00%、60.00%和30.00%。同时，喂食盐酸环丙沙星后，家蝇幼虫和蛹体内热量值均随盐酸环丙沙星喂食浓度的升高而下降（F=12.551，P<0.001）。其中，对照组幼虫和蛹体内的热量值高于0.1%、0.3%和0.5%组（t=13.093、11.294、8.601，均P<0.001）；0.1%组体内的热量值高于0.3%和0.5%组（t=10.290、7.842，均P<0.001）；0.3%组体内的热量值高于0.5%组（t=6.919，P<0.001）。结论盐酸环丙沙星可降低家蝇幼虫和蛹体内蛋白质、脂肪和总糖等能量物质的合成，降低其体内的热量值，从而影响家蝇个体发育和种群繁衍。

关键词: 家蝇, 盐酸环丙沙星, 蛋白质, 脂肪, 总糖, 热量值

Abstract: Objective To investigate the effect of ciprofloxacin hydrochloride on the content of energy substances in the larvae and pupae of Musca domestica. Methods From June to September, 2013, the laboratory population of M. domestica was used for the experiment. The newly hatched larvae were divided into control group (fed with normal forage) and three experimental groups (treated with forage containing 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride, respectively). The grinding method was used for the sampling of energy substances in the 1st instar larvae, and hemolymph was collected from the 2nd and 3rd instar larvae and pupae. The Coomassie brilliant blue method was used to measure the content of proteins, anthrone-sulfuric acid colorimetry was used to measure the content of total sugars, and phosphoric acid-vanillin chromogenic reaction was used to measure the content of lipids. SPSS 20.0 software was used for statistical analysis; a one-way analysis of variance was used for comparison between groups; the t-test was used for comparison between two groups. Results The content of proteins in the larvae and pupae in the 0.5% ciprofloxacin hydrochloride group decreased to a maximum of 4.00% and 10.00% of that in the control group; the content of lipids in the larvae in the 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride groups decreased to 75.00%, 65.00%, and 50.00% of that in the control group, while the content of lipids in the pupae in the 0.1%, 0.3%, and 0.5% groups decreased to 85.00%, 75.00%, and 50.00% of that in the control group; the content of total sugars in the 0.1%, 0.3%, and 0.5% groups decreased to 80%, 60%, and 30% of that in the control group. After the larvae and pupae of M. domestica were fed with ciprofloxacin hydrochloride, their caloric values decreased with the increase in the concentration of ciprofloxacin hydrochloride (F=12.551, P<0.001). The control group had higher caloric values of the larvae and pupae than the 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride groups (t=13.093, 11.294, 8.601, all P<0.001); the 0.1% group had higher caloric values than the 0.3% and 0.5% ciprofloxacin hydrochloride groups (t=10.290, 7.842, all P<0.001); the 0.3% group had higher caloric values than the 0.5% group (t=6.919, P<0.001). Conclusion Ciprofloxacin hydrochloride can reduce the synthesis of energy substances (proteins, lipids, and total sugars) in the larvae and pupae of M. domestica and thus reduce their caloric values, thus effects the individual development and population multiplication of M. domestica.

Key words: Musca domestica, Ciprofloxacin hydrochloride, Protein, Lipid, Total sugar, Caloric value

中图分类号:

Q965
R384.2

万晴, 宋暖, 黄振东, 薛志静, 庄桂芬, 张瑞玲, 许永玉, 张忠. 盐酸环丙沙星对家蝇幼虫和蛹能量物质含量的影响[J]. 中国媒介生物学及控制杂志, 2019, 30(5): 514-518.

WAN Qing, SONG Nuan, HUANG Zhen-dong, XUE Zhi-jing, ZHUANG Gui-fen, ZHANG Rui-ling, XU Yong-yu, ZHANG Zhong. Effect of ciprofloxacin hydrochloride on energy substances in the larvae and pupae of Musca domestica[J]. Chines Journal of Vector Biology and Control, 2019, 30(5): 514-518.

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盐酸环丙沙星对家蝇幼虫和蛹能量物质含量的影响

Effect of ciprofloxacin hydrochloride on energy substances in the larvae and pupae of Musca domestica

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