氯氰菊酯和残杀威亚致死剂量对致倦库蚊羧酸酯酶活性的影响

摘要/Abstract

摘要：

目的研究氯氰菊酯和残杀威亚致死剂量处理后致倦库蚊羧酸酯酶活性的变化规律。方法采用紫外可见分光光度计活体测定酶的活性。结果处理24 h,抗性种群致倦库蚊羧酸酯酶比活力是敏感品系的1.19倍;敏感品系处理48 h后,处理组均表现出显著的抑制作用,且与24 h相比,对照组、残杀威LC₂₀和LC₄₀处理组、氯氰菊酯LC₂₀和LC₄₀处理组的比活力分别下降24.71%、38.42%、97.42%、90.77%和95.76%,处理组比活力下降率均比对照组高。抗性种群处理48 h后,除残杀威LC₂₀ 处理组与对照组无统计学意义外,其他3个处理组均有统计学意义,残杀威LC₄₀ 处理组比活力为11.6254 nmol/(mgpro·min),表现为显著抑制作用,氯氰菊酯LC₂₀和LC₄₀处理组比活力分别为55.8868和54.5530 nmol/(mgpro·min),与对照组相比表现出显著的诱导作用,且2个浓度处理组间差异无统计学意义。比活力与24 h相比,除氯氰菊酯LC₂₀处理组比活力增加23.89%外,对照组、残杀威LC₂₀和LC₄₀处理组、氯氰菊酯LC₄₀处理组的比活力分别下降60.71%、59.14%、23.68%和47.87%,处理组比活力下降率均比对照组低。结论经氯氰菊酯和残杀威亚致死剂量在不同时间处理的敏感和抗性致倦库蚊羧酸酯酶比活力影响是不同的,表明不同药剂对同种昆虫羧酸酯酶的抑制作用不同,同一种药剂对不同种群的抑制作用也不相同。

关键词: 致倦库蚊, 羧酸酯酶, 亚致死剂量, 抗药性

Abstract:

Objective To determine the changes of carboxylesterase (CarE) activity in Culex pipiens quinqusfasciatus treated with cypermethrin and propoxur at sublethal doses. Methods The enzyme activities were measured in vitro with an ultraviolet-visible spectrophotometer. Results After 24 h treatment, the enzymatic activity of resistant strains was 1.19 times that of sensitive strains. Significant inhibition was noted in all treatment groups after 48 h. Compared with the 24 h treatment group, the enzymatic activity of the control group, propoxur at LC₂₀, propoxur at LC₄₀, cypermethrin at LC₂₀, and cypermethrin at LC₄₀ decreased by 24.71%, 38.42%, 97.42%, 90.77% and 95.76%, respectively. The decreases in all treated groups were more significant than in the control group. Activities in resistant strains treated with cypermethrin and propoxur at sublethal doses for 48 h were significantly different from the control group, except for the group treated with LC₂₀ propoxur. Compared with the control group, the specific activity was 11.6254 nmol/(mgpro·min) in the group treated with LC₄₀ of propoxur, indicating significant inhibitory effect. In the groups treated with LC₂₀ and LC₄₀ of cypermethrin, the activity was 55.8868 and 54.5530 nmol/ (mgpro·min), respectively, suggesting significant inductive effects. No difference was noted between the two 24 h and 48 h treatment groups. Compared with the 24 h treatment group, the specific activity of the control group, propoxur (at LC₂₀ and LC₄₀) and cypermethrin (at LC₄₀) treated groups decreased by 60.71% , 59.14% , 23.68% and 47.87% , respectively, whereas the cypermethrin LC₂₀ group increased by 23.89%. The decrease in the control group was lower than those in the treatment groups. Conclusion Different levels of carboxylesterase activity were observed in sensitive and resistant Cx. pipiens quinqusfasciatus when treated with sublethal doses of cypermethrin and propoxur. Thus, indicating their different inhibitory effects and that a single insecticidal agent could have different levels of activity in different populations.

Key words: Culex pipiens quinqusfasciatus, Carboxylesterase, Sublethal dose, Insecticide resistance

中图分类号:

R384.1

柳小青, 刘仰青, 熊志伟, 马红梅. 氯氰菊酯和残杀威亚致死剂量对致倦库蚊羧酸酯酶活性的影响[J]. 中国媒介生物学及控制杂志, 2011, 22(4): 309-312.

LIU Xiao-qing, LIU Yang-qing, XIONG Zhi-wei, MA Hong-mei. Effects of cypermethrin and propoxur at sublethal doses on the carboxylesterase activity in Culex pipiens quinqusfasciatus[J]. Chines Journal of Vector Biology and Control, 2011, 22(4): 309-312.

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氯氰菊酯和残杀威亚致死剂量对致倦库蚊羧酸酯酶活性的影响

Effects of cypermethrin and propoxur at sublethal doses on the carboxylesterase activity in Culex pipiens quinqusfasciatus

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