关键词: 毒力测定, 筛选, SAS, 程序, 熏蒸

Abstract:

Objective A computer program was formulated on the internationally recognized platform of statistics analysis system (SAS) for rapid screening of pesticide toxicity. Methods Two insecticides, A and B, were tested for the toxicity against Blattella germanica using indoor fumigation. The resulting data were subject to computation by this program to fit a weighted probit regression line. The LC₅₀ and 95% confidence interval (95%CI) of both agents were then calculated and compared in pair with the slope of the toxicity regression line. Results The toxicity regression lines of the two agents were Y(probit)＝ -3.193＋5.005×Log₁₀ (dose) and Y(probit)＝-2.498＋2.696×Log₁₀ (dose). The Wald test showed both P values were ＜0.01, while the goodness-of-fit test showed both P values were ＞0.05. The LC₅₀ values for the two pesticides were 4.35 g/m³ (95%CI: 3.17-5.29 g/m³) and 8.44 g/m³ (95%CI: 6.07-10.66 g/m³), respectively; the slopes of the two regression lines were 5.005 and 2.696, respectively. Comparison between the LC₅₀ values showed: u＝3.415, P＜0.001; and that between slopes revealed: t＝2.028, P＜ 0.05. These results suggested that agent A was stronger than B in both toxicity and sensitivity. Conclusion Through dual verification, the established method and computer program proved to be accurate, reliable, and simple and fast for rapid screening of insecticidal toxicity.

Key words: Toxicity measurement, Screening, Statistics analysis system, Program, Fumigation